| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Acetomicrobium | CCAAGACACCATGCAACGG | AGGCCAAATTTTCTGCGCA | 59.42 | 58.95 | 201 | 124 |
100.00%
|
100.00%
|
| Primer 2 | Acetomicrobium | CCAAGACACCATGCAACGG | TCCTCCTTCGATTTGGCGTC | 59.42 | 60.11 | 297 | 124 |
100.00%
|
100.00%
|
| Primer 3 | Acetomicrobium | CCAAGACACCATGCAACGG | GGCGTCAAATATGGCTTCGG | 59.42 | 59.69 | 283 | 124 |
100.00%
|
100.00%
|
| Primer 4 | Acetomicrobium | CCAAGACACCATGCAACGG | GCGTCAAATATGGCTTCGGC | 59.42 | 60.32 | 282 | 124 |
100.00%
|
100.00%
|
| Primer 5 | Acetomicrobium | CCAAGACACCATGCAACGG | CCTCCTTCGATTTGGCGTC | 59.42 | 58.62 | 296 | 124 |
100.00%
|
100.00%
|
| Primer 6 | Acetomicrobium | CCAAGACACCATGCAACGG | TCCTCCTTGGATTTGGCGTC | 59.42 | 60.04 | 261 | 124 |
100.00%
|
100.00%
|
| Primer 7 | Acetomicrobium | CCAAGACACCATGCAACGG | GGCGTCGAGTTTAGCTTCG | 59.42 | 59.01 | 259 | 118 |
100.00%
|
100.00%
|
| Primer 8 | Acetomicrobium | CGATGCCTCAATTGCGGTG | TTGCACAATGGGCAGCTG | 59.94 | 58.95 | 158 | 112 |
100.00%
|
100.00%
|
| Primer 9 | Acetomicrobium | AGCGCTAGTGCCAATTGC | TGTCTTCCAGGGACATCAGC | 58.81 | 59.38 | 258 | 107 |
100.00%
|
50.00%
|
| Primer 10 | Acetomicrobium | CCAAGACACCATGCAACGG | TCCTCCTTCGATTTGGCGT | 59.42 | 59.02 | 297 | 106 |
100.00%
|
100.00%
|