PrimerBac

PrimerBac is a software that designs specific PCR primers to identify intestinal bacteria in complex environments. The website compiles the results of the software's specific primer design for most Genus and Specs in the intestine, presented in the form of charts and graphs

The Pangenome of Top 20 Phylum

Primer Process

Firstly, download bacterial genome information from the NCBI database, and then perform pan genomic analysis on the downloaded bacterial genome data. Based on the results of pan genomic analysis, identify core homologous genome genes with classification potential, design primers for them, and finally verify the specificity of primers in the gut microbiota.