| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Acholeplasma | ACAGCTGTACCGGTTGCAC | TTACGCTTGCACCACTGC | 60.60 | 58.66 | 276 | 157 |
100.00%
|
100.00%
|
| Primer 2 | Acholeplasma | TGCTGCAATGCACTTAGGTG | GAGGTTCTTGGTCTAGGGCAG | 59.12 | 59.79 | 173 | 151 |
100.00%
|
100.00%
|
| Primer 3 | Acholeplasma | AGGACTCGACGTTAAAGGGC | GCTGCGAGCTCATTTAATGTG | 59.75 | 58.29 | 234 | 143 |
100.00%
|
100.00%
|
| Primer 4 | Acholeplasma | TGCGGTGGTAGCATGTCTG | ACGCCTTCTCCCCAAAAGG | 60.08 | 59.93 | 251 | 141 |
100.00%
|
100.00%
|
| Primer 5 | Acholeplasma | ACACTCCCAACCGCATCTG | ATGTGCCTGCACCAACTG | 60.00 | 58.23 | 250 | 141 |
100.00%
|
100.00%
|
| Primer 6 | Acholeplasma | TGCTGCAATGCACTTAGGTG | AGGTTCTTGGTCTAGGGCAG | 59.12 | 58.72 | 172 | 140 |
100.00%
|
100.00%
|
| Primer 7 | Acholeplasma | GGTTGCACCAACGCCTAAC | TTACGCTTGCACCACTGC | 59.72 | 58.66 | 265 | 140 |
100.00%
|
100.00%
|
| Primer 8 | Acholeplasma | CAGTTGCCGTAGCCAAATGG | TCCAAACCCCAATCCTCGTG | 59.83 | 59.96 | 261 | 140 |
100.00%
|
100.00%
|
| Primer 9 | Acholeplasma | GGACTCGACGTTAAAGGGCA | GCTGCGAGCTCATTTAATGTG | 60.04 | 58.29 | 233 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Acholeplasma | GCTGGGCTGGATCATACCAT | ACTGCTGCAATAACCCAAACA | 59.60 | 58.68 | 294 | 137 |
100.00%
|
100.00%
|