| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Acidaminococcus | TGCTGCTTTGTGCTGTGC | AGCAGCTCTGTTTCCGGTC | 59.59 | 60.00 | 217 | 148 |
100.00%
|
50.00%
|
| Primer 2 | Acidaminococcus | TGGAACACAACACCGCTGT | TGCATGCTGCCAACCAGT | 60.08 | 60.20 | 205 | 133 |
100.00%
|
50.00%
|
| Primer 3 | Acidaminococcus | TGCCATCAAGCAGCGGAT | ACGCTCCCACTTCTTGGTG | 59.73 | 59.93 | 187 | 131 |
100.00%
|
33.33%
|
| Primer 4 | Acidaminococcus | TGCCATCAAGCAGCGGAT | AGGGTCTGCATGGCATAGC | 59.73 | 59.85 | 270 | 131 |
100.00%
|
33.33%
|
| Primer 5 | Acidaminococcus | TCCATGGCCAATTCCGCA | TTCCTGTTCCGGTTCCAGC | 59.64 | 59.93 | 243 | 128 |
100.00%
|
50.00%
|
| Primer 6 | Acidaminococcus | TCCATGGCCAATTCCGCA | TTCCAGCTGAAGGACCGTG | 59.64 | 59.63 | 171 | 128 |
100.00%
|
33.33%
|
| Primer 7 | Acidaminococcus | TGCCATCAAGCAGCGGAT | GGGTCTGCATGGCATAGCA | 59.73 | 60.15 | 269 | 128 |
100.00%
|
33.33%
|
| Primer 8 | Acidaminococcus | TCCATGGCCAATTCCGCA | TCCATGGAGGTGTTCCCCA | 59.64 | 60.15 | 206 | 125 |
100.00%
|
50.00%
|
| Primer 9 | Acidaminococcus | TGTGGAAAAGGCACGCCA | CCCATGGCAAAAGGCTTGG | 60.12 | 59.70 | 210 | 125 |
100.00%
|
50.00%
|
| Primer 10 | Acidaminococcus | TGTGGAAAAGGCACGCCA | GGCAAAAGGCTTGGTCAGG | 60.12 | 59.33 | 205 | 125 |
100.00%
|
50.00%
|