| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Acidithiobacillus | TTCAACAAGGCTGCCCTCC | AACATTGCCAAGCGCTGC | 60.23 | 60.05 | 246 | 168 |
100.00%
|
50.00%
|
| Primer 2 | Acidithiobacillus | AGCTTGGCTGCACATTGG | AACATTGCCAAGCGCTGC | 58.63 | 60.05 | 264 | 168 |
100.00%
|
50.00%
|
| Primer 3 | Acidithiobacillus | TTTTGGTGGCATCGCGTG | TGCGAGAACATCCACCAGG | 59.36 | 59.70 | 202 | 162 |
100.00%
|
50.00%
|
| Primer 4 | Acidithiobacillus | AACAAGGCTGCCCTCCAT | AACATTGCCAAGCGCTGC | 58.82 | 60.05 | 243 | 149 |
100.00%
|
50.00%
|
| Primer 5 | Acidithiobacillus | CAACAAGGCTGCCCTCCAT | AACATTGCCAAGCGCTGC | 60.30 | 60.05 | 244 | 149 |
100.00%
|
50.00%
|
| Primer 6 | Acidithiobacillus | AACAAGGCTGCCCTCCATG | AACATTGCCAAGCGCTGC | 60.30 | 60.05 | 243 | 145 |
100.00%
|
50.00%
|
| Primer 7 | Acidithiobacillus | TTCCGGAGCGTGGATGAAC | AAAGCGTTCGTCTGCGGA | 60.08 | 59.97 | 221 | 140 |
100.00%
|
50.00%
|
| Primer 8 | Acidithiobacillus | TGCTCAGTCTCGATGGTGC | AAAGCGTTCGTCTGCGGA | 59.78 | 59.97 | 151 | 140 |
100.00%
|
50.00%
|
| Primer 9 | Acidithiobacillus | TGTTCACGGTATCGGTCCC | AAAGCGTTCGTCTGCGGA | 59.11 | 59.97 | 195 | 140 |
100.00%
|
50.00%
|
| Primer 10 | Acidithiobacillus | ATGCTGGTGTTCCTGGTGG | TCCGCACGCACTTCAACT | 59.92 | 59.89 | 151 | 138 |
100.00%
|
50.00%
|