| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Anaerococcus | TGAAGGTGCAATCGGTGGAG | GCTGGGCAAATCTTTGGAGC | 60.32 | 60.11 | 267 | 148 |
100.00%
|
35.71%
|
| Primer 2 | Anaerococcus | GAAGGTGCAATCGGTGGAG | GCTGGGCAAATCTTTGGAGC | 58.53 | 60.11 | 266 | 148 |
100.00%
|
35.71%
|
| Primer 3 | Anaerococcus | TGAAGGTGCAATCGGTGGA | GCTGGGCAAATCTTTGGAGC | 59.24 | 60.11 | 267 | 140 |
100.00%
|
35.71%
|
| Primer 4 | Anaerococcus | TTGAAGGTGCAATCGGTGGA | GCTGGGCAAATCTTTGGAGC | 59.89 | 60.11 | 268 | 140 |
100.00%
|
35.71%
|
| Primer 5 | Anaerococcus | AGGTGCAATCGGTGGAGAAA | GCTGGGCAAATCTTTGGAGC | 59.60 | 60.11 | 264 | 137 |
100.00%
|
50.00%
|
| Primer 6 | Anaerococcus | GCTAACCAAATCGAGGCTGC | GCGCTGCAAGTCTCATAGC | 59.62 | 59.36 | 238 | 135 |
100.00%
|
35.71%
|
| Primer 7 | Anaerococcus | TGGGCAAGTGCTGGACAA | ACTTCAAGCCCTGCTGCT | 59.40 | 59.16 | 191 | 128 |
100.00%
|
35.71%
|
| Primer 8 | Anaerococcus | AGGTGCAATCGGTGGAGAA | GCTGGGCAAATCTTTGGAGC | 58.93 | 60.11 | 264 | 128 |
100.00%
|
50.00%
|
| Primer 9 | Anaerococcus | AAGGTGCAATCGGTGGAGAA | GCTGGGCAAATCTTTGGAGC | 59.60 | 60.11 | 265 | 128 |
100.00%
|
50.00%
|
| Primer 10 | Anaerococcus | AGCTAGCCAAGTTGCAGCA | GTTGTGTGGAAGCATGCCC | 59.93 | 59.71 | 253 | 127 |
100.00%
|
35.71%
|