| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Barnesiella | ACTGCCGTCCCGAAATACC | AAACGGAGTCCATCGGCTG | 59.78 | 60.08 | 255 | 139 |
100.00%
|
100.00%
|
| Primer 2 | Barnesiella | TGCTTTGAACGTGCGTGC | ATGGTCGGGAATACGCACC | 59.98 | 59.85 | 247 | 104 |
100.00%
|
100.00%
|
| Primer 3 | Barnesiella | TGCTTCGTTGACGGTTGC | ATGTAACCACCCACGGCAG | 58.98 | 60.00 | 297 | 104 |
100.00%
|
100.00%
|
| Primer 4 | Barnesiella | AGTCGACTAAACAGCGGACA | AAGCATCGGTCGCTGCAT | 59.04 | 60.12 | 205 | 101 |
100.00%
|
100.00%
|
| Primer 5 | Barnesiella | CCGCACAGTTTGACGGTTC | ACGGAGCCTTTCGCAACA | 59.72 | 59.89 | 154 | 101 |
100.00%
|
100.00%
|
| Primer 6 | Barnesiella | GGGTTATTTCGCCTGTGGC | ACGGAGCCTTTCGCAACA | 59.19 | 59.89 | 174 | 101 |
100.00%
|
100.00%
|
| Primer 7 | Barnesiella | TCGAACTGCACGATGCCA | ACATGCGGTCGGTCATGT | 59.66 | 59.34 | 231 | 99 |
100.00%
|
100.00%
|
| Primer 8 | Barnesiella | TCGCAAGCGATGGAGCAT | GCGCAGAAAGGAACCCAAC | 59.81 | 59.71 | 202 | 99 |
100.00%
|
100.00%
|
| Primer 9 | Barnesiella | TTGCGGTGAAGCCTTCCA | CTTTTCGTCGGTCGTCCCA | 59.49 | 60.01 | 232 | 99 |
100.00%
|
100.00%
|
| Primer 10 | Barnesiella | TTGCGGTGAAGCCTTCCA | TTTTCGTCGGTCGTCCCA | 59.49 | 58.87 | 231 | 99 |
100.00%
|
100.00%
|