| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bradyrhizobium | AGATCGGCATGCTCCTGTG | ATCATGCCGTCGACGAAGG | 59.85 | 60.23 | 163 | 81 |
100.00%
|
66.67%
|
| Primer 2 | Bradyrhizobium | AACGCTGGCTCACCTCAT | TGCAGACCTTGGCGTTGT | 58.93 | 59.81 | 179 | 72 |
100.00%
|
66.67%
|
| Primer 3 | Bradyrhizobium | AACGCTGGCTCACCTCATC | TGCAGACCTTGGCGTTGT | 60.08 | 59.81 | 179 | 72 |
100.00%
|
66.67%
|
| Primer 4 | Bradyrhizobium | GAACGCTGGCTCACCTCAT | TGCAGACCTTGGCGTTGT | 60.08 | 59.81 | 180 | 72 |
100.00%
|
66.67%
|
| Primer 5 | Bradyrhizobium | ACCTCGACGCATTTCCTGG | TCGAACAGCGAGCCGAAA | 60.08 | 59.66 | 263 | 61 |
100.00%
|
66.67%
|
| Primer 6 | Bradyrhizobium | CCTCGACGCATTTCCTGGA | TCGAACAGCGAGCCGAAA | 59.78 | 59.66 | 262 | 61 |
100.00%
|
66.67%
|
| Primer 7 | Bradyrhizobium | CACCTGTGCTCGCTGATGA | GCGCACGGTGCATTTGAA | 60.08 | 60.05 | 207 | 60 |
100.00%
|
66.67%
|
| Primer 8 | Bradyrhizobium | AGTTCGATCCGCAACCGAT | GCGCACGGTGCATTTGAA | 59.48 | 60.05 | 287 | 60 |
100.00%
|
66.67%
|
| Primer 9 | Bradyrhizobium | GTTCGATCCGCAACCGATG | GCGCACGGTGCATTTGAA | 59.36 | 60.05 | 286 | 60 |
100.00%
|
66.67%
|
| Primer 10 | Bradyrhizobium | CTGTGCTCGCTGATGATGC | GCGCACGGTGCATTTGAA | 59.36 | 60.05 | 204 | 60 |
100.00%
|
66.67%
|