| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Butyrivibrio | GACCAAACGCATGTCCACC | TCACGGCAACAGGAAGTCC | 59.42 | 59.93 | 264 | 112 |
100.00%
|
50.00%
|
| Primer 2 | Butyrivibrio | ACCAAACGCATGTCCACC | TCACGGCAACAGGAAGTCC | 58.24 | 59.93 | 263 | 112 |
100.00%
|
50.00%
|
| Primer 3 | Butyrivibrio | GCCAGATGCATTCCGATGG | TGCTAACGGCTCACCAAGG | 59.05 | 60.00 | 261 | 109 |
100.00%
|
50.00%
|
| Primer 4 | Butyrivibrio | ACGGAGTTGACACAGGATGG | TGCTAACGGCTCACCAAGG | 59.68 | 60.00 | 236 | 102 |
100.00%
|
50.00%
|
| Primer 5 | Butyrivibrio | GCACCGACAACGAAACCAC | AACCGGTGCTGTTGTTGC | 60.01 | 59.20 | 192 | 101 |
100.00%
|
50.00%
|
| Primer 6 | Butyrivibrio | GGACCAAACGCATGTCCAC | TCACGGCAACAGGAAGTCC | 59.42 | 59.93 | 265 | 99 |
100.00%
|
50.00%
|
| Primer 7 | Butyrivibrio | TTCGGAAGTACACGGCAGG | TCCGCATACTCACTGCCTG | 59.71 | 59.48 | 174 | 98 |
100.00%
|
50.00%
|
| Primer 8 | Butyrivibrio | TCCAAGCCGACAGTAGAGC | CGAAAGCACGGAACATGACC | 59.11 | 59.83 | 247 | 96 |
100.00%
|
50.00%
|
| Primer 9 | Butyrivibrio | TTGCGTTGCGTGAGTTGG | GCTCCCCACCTTAGGATTGG | 59.29 | 59.82 | 158 | 96 |
100.00%
|
50.00%
|
| Primer 10 | Butyrivibrio | TGGCAAGGGTAGCACACAG | TCGAAAGCTTTGCCGTGG | 59.93 | 58.66 | 283 | 94 |
100.00%
|
50.00%
|