| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Caldicellulosiruptor | TGAGGCGTGTGGACTTCTG | GGCCTTGAAGGTGATGTTGG | 59.63 | 59.11 | 204 | 97 |
100.00%
|
50.00%
|
| Primer 2 | Caldicellulosiruptor | GCACGGTAAGAGAGATTCAGG | GCACTGTTTTGTAGCCGTCAG | 58.18 | 60.07 | 168 | 95 |
100.00%
|
50.00%
|
| Primer 3 | Caldicellulosiruptor | TCAAACTGGTGCTGGGCAG | CGGCAAAATGTCCGGTTCC | 60.53 | 59.79 | 298 | 92 |
100.00%
|
50.00%
|
| Primer 4 | Caldicellulosiruptor | GGTATTTAGCAGGTGCAGAGG | TTCTGCTCTTAGCCCTCTCC | 58.43 | 58.51 | 183 | 91 |
100.00%
|
50.00%
|
| Primer 5 | Caldicellulosiruptor | TCTCGACGCACTTGAGCTG | GAAGGCGTTGAGGAATGACC | 60.08 | 58.91 | 160 | 90 |
100.00%
|
50.00%
|
| Primer 6 | Caldicellulosiruptor | GTTTGGACACATCAAGCCCAG | TATCCCAGCAGCACCAAGC | 59.73 | 60.08 | 218 | 90 |
100.00%
|
50.00%
|
| Primer 7 | Caldicellulosiruptor | GCACGGTAAGAGAGATTCAGG | CACTGTTTTGTAGCCGTCAGC | 58.18 | 60.07 | 167 | 90 |
100.00%
|
50.00%
|
| Primer 8 | Caldicellulosiruptor | GCACGGTAAGAGAGATTCAGG | ACTGTTTTGTAGCCGTCAGC | 58.18 | 58.77 | 166 | 90 |
100.00%
|
50.00%
|
| Primer 9 | Caldicellulosiruptor | TCTCGACGCACTTGAGCTG | AGAAGGCGTTGAGGAATGACC | 60.08 | 60.34 | 161 | 88 |
100.00%
|
50.00%
|
| Primer 10 | Caldicellulosiruptor | TGCTGAGGTCACTTGACAGG | TATTCGCACCGTACCGCAG | 59.61 | 60.23 | 264 | 87 |
100.00%
|
50.00%
|