| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Citrobacter | ATGAGTATGCGACCGAGGC | ACCCCAGCGATTGAGGAAC | 59.64 | 59.70 | 197 | 143 |
100.00%
|
37.50%
|
| Primer 2 | Citrobacter | TGCATGGCGTTTTCCGGA | GCCGATTTCCTGCATGCAG | 60.28 | 59.93 | 151 | 140 |
100.00%
|
68.75%
|
| Primer 3 | Citrobacter | TGCATGGCGTTTTCCGGA | AGTTCGCTGGTACGCACT | 60.28 | 58.95 | 231 | 138 |
100.00%
|
56.25%
|
| Primer 4 | Citrobacter | ATGCTTCCCTGGGATTGGC | AACACTCCGCATGACGCA | 60.08 | 59.97 | 276 | 138 |
100.00%
|
37.50%
|
| Primer 5 | Citrobacter | ATCCCAACGGACAGAGTGC | AACACTCCGCATGACGCA | 59.70 | 59.97 | 175 | 138 |
100.00%
|
50.00%
|
| Primer 6 | Citrobacter | ACGGACAGAGTGCACGTTC | AACACTCCGCATGACGCA | 60.30 | 59.97 | 169 | 138 |
100.00%
|
50.00%
|
| Primer 7 | Citrobacter | TCCCTGGGATTGGCCTACT | AACACTCCGCATGACGCA | 59.60 | 59.97 | 271 | 138 |
100.00%
|
43.75%
|
| Primer 8 | Citrobacter | TCGCAAGCTGTCAGCAGT | GATTGGTCTGCAGGCGACT | 59.58 | 60.08 | 151 | 138 |
100.00%
|
37.50%
|
| Primer 9 | Citrobacter | ATCCCAACGGACAGAGTGC | AACACTCCGCATGACGCA | 59.70 | 59.97 | 175 | 138 |
100.00%
|
50.00%
|
| Primer 10 | Citrobacter | ACGGACAGAGTGCACGTTC | AACACTCCGCATGACGCA | 60.30 | 59.97 | 169 | 138 |
100.00%
|
50.00%
|