| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Cloacibacillus | ACGCATTCAACCGCAAGC | TCGGAAGTAAAGGGCAGCAG | 59.74 | 60.04 | 229 | 138 |
100.00%
|
50.00%
|
| Primer 2 | Cloacibacillus | ACGCATTCAACCGCAAGC | GGAAGTAAAGGGCAGCAGC | 59.74 | 58.82 | 227 | 138 |
100.00%
|
50.00%
|
| Primer 3 | Cloacibacillus | ACGCATTCAACCGCAAGC | CGGAAGTAAAGGGCAGCAG | 59.74 | 58.53 | 228 | 138 |
100.00%
|
50.00%
|
| Primer 4 | Cloacibacillus | ACGCATTCAACCGCAAGC | AGTCGGATGTGAAGGGCAG | 59.74 | 59.40 | 231 | 138 |
100.00%
|
50.00%
|
| Primer 5 | Cloacibacillus | ATCGGCAAGGTACAGGTGG | TGTCTTGAGTGACGGCAGG | 59.40 | 59.63 | 183 | 127 |
100.00%
|
50.00%
|
| Primer 6 | Cloacibacillus | ATTCCAGTGTCCCTGCTGC | ATGGACGAAAGAGCGCTCC | 60.00 | 60.15 | 280 | 122 |
100.00%
|
50.00%
|
| Primer 7 | Cloacibacillus | ACGAAGGAAGAGGCGTTGG | AAACGGCTCGACCTCTTCC | 60.00 | 59.71 | 282 | 120 |
100.00%
|
50.00%
|
| Primer 8 | Cloacibacillus | TTTCTGACCCTGCGGACAC | TGACGACGACCATCCAAGC | 59.93 | 60.08 | 244 | 115 |
100.00%
|
50.00%
|
| Primer 9 | Cloacibacillus | AAGGAAGAGGCGTTGGAGC | AAACGGCTCGACCTCTTCC | 60.00 | 59.71 | 279 | 114 |
100.00%
|
50.00%
|
| Primer 10 | Cloacibacillus | TGTCAAAGGGGAGTGGCTG | TGACCGAAACGCTGCAGT | 59.54 | 59.89 | 204 | 112 |
100.00%
|
50.00%
|