| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Dialister | ATCAGTCCGGAATCAGCACC | TGGTTTCAGGCTGTACGGC | 59.82 | 60.30 | 151 | 88 |
100.00%
|
50.00%
|
| Primer 2 | Dialister | TGCGGACCTTTGCTGGAA | TGCCTTCCTGAATGCGCA | 59.49 | 59.97 | 233 | 72 |
100.00%
|
50.00%
|
| Primer 3 | Dialister | TTGCGGACCTTTGCTGGA | TGCCTTCCTGAATGCGCA | 59.49 | 59.97 | 234 | 71 |
100.00%
|
50.00%
|
| Primer 4 | Dialister | ACCTGAGCACTGCCGAAA | TGGCAGCCTTCAATTCGTCT | 59.17 | 59.96 | 174 | 60 |
100.00%
|
50.00%
|
| Primer 5 | Dialister | AACCTGAGCACTGCCGAA | TGGCAGCCTTCAATTCGTCT | 59.17 | 59.96 | 175 | 60 |
100.00%
|
50.00%
|
| Primer 6 | Dialister | AACCTGAGCACTGCCGAA | CCTTCTTGGCAGCCTTCAAT | 59.17 | 58.45 | 181 | 55 |
100.00%
|
50.00%
|
| Primer 7 | Dialister | TGAACGCTTCTGCCCAGT | AGCACCGACGGCAACTTT | 59.17 | 60.20 | 260 | 38 |
100.00%
|
50.00%
|