| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Eikenella | GCAACATCCGTGCATCCAC | ATGGCCAGCAGCTCTTCTG | 59.86 | 60.08 | 171 | 149 |
100.00%
|
50.00%
|
| Primer 2 | Eikenella | GCAACATCCGTGCATCCAC | TGATGGCCAGCAGCTCTTC | 59.86 | 60.08 | 173 | 139 |
100.00%
|
50.00%
|
| Primer 3 | Eikenella | CGCTTCGCTGCTGCAAAA | GGTGATTTGCAGGTCGGGA | 60.05 | 60.00 | 202 | 127 |
100.00%
|
50.00%
|
| Primer 4 | Eikenella | CGCTTCGCTGCTGCAAAA | TAACGTTGCCCAAGCCCA | 60.05 | 59.48 | 275 | 125 |
100.00%
|
50.00%
|
| Primer 5 | Eikenella | ACGGCTTCGTGTTGCTGA | TGCTGCTGCCGTTTTGAG | 59.89 | 58.97 | 177 | 122 |
100.00%
|
50.00%
|
| Primer 6 | Eikenella | ACGGCTTCGTGTTGCTGA | GCTCTTCAATGCTGCTGCC | 59.89 | 59.86 | 186 | 122 |
100.00%
|
50.00%
|
| Primer 7 | Eikenella | ACGGCTTCGTGTTGCTGA | CTCTTCAATGCTGCTGCCG | 59.89 | 59.57 | 185 | 122 |
100.00%
|
50.00%
|
| Primer 8 | Eikenella | CGCTTCGCTGCTGCAAAA | TTTGCTGCAGGAATGCGG | 60.05 | 59.04 | 245 | 120 |
100.00%
|
50.00%
|
| Primer 9 | Eikenella | ACCGTCTTGATTGAGCGCA | AGCAGGCTTTGAACAACCC | 60.01 | 58.57 | 173 | 118 |
100.00%
|
50.00%
|
| Primer 10 | Eikenella | CGCTTCGCTGCTGCAAAA | TTGCTGCAGGAATGCGGT | 60.05 | 60.28 | 244 | 118 |
100.00%
|
50.00%
|