| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Enterococcus | ACCACTGCCGAAATGCTTG | GCTTGTTCACGCAACACAGT | 59.04 | 59.90 | 155 | 121 |
100.00%
|
30.43%
|
| Primer 2 | Enterococcus | ACCACTGCCGAAATGCTTG | AGCTTGTTCACGCAACACAG | 59.04 | 59.62 | 156 | 120 |
100.00%
|
28.26%
|
| Primer 3 | Enterococcus | ACCACTGCCGAAATGCTTG | TCGCTTGTTCACGCAACAC | 59.04 | 59.65 | 157 | 109 |
100.00%
|
30.43%
|
| Primer 4 | Enterococcus | ACCACTGCCGAAATGCTTG | AGCTTGTTCACGCAACACAG | 59.04 | 59.62 | 156 | 108 |
100.00%
|
28.26%
|
| Primer 5 | Enterococcus | ACCACTGCCGAAATGCTTG | GCTTGTTCACGCAACACAGT | 59.04 | 59.90 | 155 | 107 |
100.00%
|
30.43%
|
| Primer 6 | Enterococcus | ACCACTGCCGAAATGCTAGA | GCTTGTTCACGCAACACAGT | 59.39 | 59.90 | 155 | 103 |
100.00%
|
30.43%
|
| Primer 7 | Enterococcus | TCGAAGGAACTCGCGAACG | ACGAGCTGCTTTTCCGTGT | 60.44 | 60.23 | 230 | 99 |
100.00%
|
30.43%
|
| Primer 8 | Enterococcus | AACACCACCAGCTGCAGT | AACCATGCGCATTGCTGC | 59.40 | 60.13 | 172 | 97 |
100.00%
|
30.43%
|
| Primer 9 | Enterococcus | TGAGTGCCCACGCTTGAA | TCCATTTTCGCGAGCTGC | 59.49 | 58.82 | 268 | 95 |
100.00%
|
23.91%
|
| Primer 10 | Enterococcus | ACGCTAGCAAGTGCCTCTG | TCCATTTTCGCGAGCTGC | 60.08 | 58.82 | 192 | 93 |
100.00%
|
23.91%
|