| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Francisella | TGTTGTCGTTGTCGCTGC | CGCCAAACATTCCTGCAGC | 58.99 | 60.44 | 212 | 157 |
100.00%
|
50.00%
|
| Primer 2 | Francisella | ATGGGTGTGGGTGATGCTG | ACATACGATCGCCACGTAGC | 60.00 | 60.32 | 178 | 154 |
100.00%
|
50.00%
|
| Primer 3 | Francisella | ATGGGTGTGGGTGATGCTG | CATACGATCGCCACGTAGC | 60.00 | 58.58 | 177 | 143 |
100.00%
|
50.00%
|
| Primer 4 | Francisella | TGGGTGGTTCTCATCGCAG | AGAAGCACGGCAGCATTG | 59.70 | 58.73 | 187 | 142 |
100.00%
|
50.00%
|
| Primer 5 | Francisella | AGGTGAGGGTGCAGCATTG | AATGCTGCATCGGCTGGA | 60.30 | 59.73 | 252 | 140 |
100.00%
|
50.00%
|
| Primer 6 | Francisella | TAGGTGAGGGTGCAGCATTG | AATGCTGCATCGGCTGGA | 60.04 | 59.73 | 253 | 140 |
100.00%
|
50.00%
|
| Primer 7 | Francisella | AGTCGAAGCCGTAAGACAGC | AATGCTGCATCGGCTGGA | 60.11 | 59.73 | 198 | 140 |
100.00%
|
50.00%
|
| Primer 8 | Francisella | ACGCACCAACTGAAGCTG | AAAGCATCCGCAGCATCC | 58.26 | 58.48 | 256 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Francisella | AATGGCGTGGGTTTAGCAG | CCTTTGCGGAAACACCAGG | 58.43 | 59.34 | 181 | 137 |
100.00%
|
50.00%
|
| Primer 10 | Francisella | ACATTCCCAGCTTTGGCAG | AGGGCTAGGTTCGGAGTCA | 58.34 | 59.61 | 267 | 132 |
100.00%
|
50.00%
|