| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Gemella | AGCCAAGGGTTCAACGCA | CTCCAAGCGCTTCTGGTGT | 59.81 | 60.30 | 265 | 128 |
100.00%
|
50.00%
|
| Primer 2 | Gemella | AGCCAAGGGTTCAACGCA | ACTCCAAGCGCTTCTGGTG | 59.81 | 60.30 | 266 | 128 |
100.00%
|
50.00%
|
| Primer 3 | Gemella | AGCCAAGGGTTCAACGCA | TCCAAGCGCTTCTGGTGTAG | 59.81 | 60.04 | 264 | 128 |
100.00%
|
50.00%
|
| Primer 4 | Gemella | AGCCAAGGGTTCAACGCA | TACTCCAAGCGCTTCTGGTG | 59.81 | 60.04 | 267 | 128 |
100.00%
|
50.00%
|
| Primer 5 | Gemella | GCCCTTCTATCTAACCCTCGC | TCCTTCGTGTTGCGCTCT | 60.00 | 59.27 | 240 | 125 |
100.00%
|
50.00%
|
| Primer 6 | Gemella | AGCCAAGGGTTCAACGCA | CTCCAAGCGCTTCTGGTGTA | 59.81 | 60.04 | 265 | 123 |
100.00%
|
50.00%
|
| Primer 7 | Gemella | AGCCAAGGGTTCAACGCA | GTACTCCAAGCGCTTCTGGT | 59.81 | 60.04 | 268 | 118 |
100.00%
|
50.00%
|
| Primer 8 | Gemella | AGCACGTAGTTTTGGGGACT | AGCTTCCGCACTACCTACA | 59.24 | 58.03 | 229 | 117 |
100.00%
|
50.00%
|
| Primer 9 | Gemella | AGCCAAGGGTTCAACGCA | CCAAGCGCTTCTGGTGTAGA | 59.81 | 60.04 | 263 | 115 |
100.00%
|
50.00%
|
| Primer 10 | Gemella | CTCGCCAAATCGAAGCTGC | GCAACCCATCCTTCAACTGC | 59.94 | 59.76 | 154 | 112 |
100.00%
|
50.00%
|