| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Kurthia | CGAAAGATCGCCAACCATTTG | CCGCATCTGCTTGTGTTGC | 58.48 | 60.44 | 168 | 143 |
100.00%
|
50.00%
|
| Primer 2 | Kurthia | CGAAAGATCGCCAACCATTTG | TGTCCGCATCTGCTTGTG | 58.48 | 58.03 | 171 | 143 |
100.00%
|
50.00%
|
| Primer 3 | Kurthia | CGAAAGATCGCCAACCATTTG | GTCCGCATCTGCTTGTGTTG | 58.48 | 60.11 | 170 | 143 |
100.00%
|
50.00%
|
| Primer 4 | Kurthia | TGTGACGCAAGCACAAGC | AGACCGGTCCACCTGTTTTC | 59.28 | 59.89 | 254 | 143 |
100.00%
|
50.00%
|
| Primer 5 | Kurthia | CGAAAGATCGCCAACCATTTG | TGTCCGCATCTGCTTGTGT | 58.48 | 59.93 | 171 | 138 |
100.00%
|
50.00%
|
| Primer 6 | Kurthia | CGAAAGATCGCCAACCATTTG | GTCCGCATCTGCTTGTGT | 58.48 | 58.03 | 170 | 138 |
100.00%
|
50.00%
|
| Primer 7 | Kurthia | ACTTGGGAAACGGGTGTCC | TGCGACGCAAATCCATGC | 59.85 | 59.52 | 246 | 138 |
100.00%
|
50.00%
|
| Primer 8 | Kurthia | ATGTGTGGCAACGAGAGC | CGCGTCCATTTCCTTCTGC | 58.03 | 59.57 | 161 | 138 |
100.00%
|
50.00%
|
| Primer 9 | Kurthia | AGGGGTTCGGAGGAGATGT | TCGTTGCAGCTTCTGAAGTG | 59.61 | 58.78 | 186 | 138 |
100.00%
|
50.00%
|
| Primer 10 | Kurthia | AGGGGTTCGGAGGAGATGT | TGCAGCTTCTGAAGTGAACC | 59.61 | 58.40 | 182 | 138 |
100.00%
|
50.00%
|