| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lactiplantibacillus | TCCCTGATTTCCGTGCTGG | AATTGCAGCAACGCGTGG | 59.70 | 60.05 | 203 | 167 |
100.00%
|
66.67%
|
| Primer 2 | Lactiplantibacillus | TTAGCGGTTGGGATGGCTG | TTCGTACTGCACCAGCTGG | 60.08 | 60.01 | 243 | 152 |
100.00%
|
33.33%
|
| Primer 3 | Lactiplantibacillus | GCGTTTGCCTGCTCATTGT | TCAGGCCTTTCTCACGCAC | 59.71 | 60.30 | 187 | 148 |
100.00%
|
33.33%
|
| Primer 4 | Lactiplantibacillus | GCGTTTGCCTGCTCATTGT | GGCCTTTCTCACGCACCAT | 59.71 | 60.68 | 184 | 148 |
100.00%
|
33.33%
|
| Primer 5 | Lactiplantibacillus | TGTACCGCTGATGCCGATG | ACGCATCTGCCATGCTGT | 60.23 | 60.05 | 182 | 145 |
100.00%
|
66.67%
|
| Primer 6 | Lactiplantibacillus | AAACGCTGGGTGTGCAGT | CCGCTGAATTGGCTTCGTG | 60.12 | 59.86 | 210 | 143 |
100.00%
|
33.33%
|
| Primer 7 | Lactiplantibacillus | CGGACTCAACGTGACGGAT | TGTGGCATTGTCCGCTGT | 59.79 | 59.89 | 199 | 141 |
100.00%
|
33.33%
|
| Primer 8 | Lactiplantibacillus | CATGGGAGTTTTGCGCAGG | ATCAGACCCATGCGCTCTG | 59.79 | 59.85 | 245 | 141 |
100.00%
|
33.33%
|
| Primer 9 | Lactiplantibacillus | TGTACCGCTGATGCCGAT | ACGCATCTGCCATGCTGT | 58.78 | 60.05 | 182 | 141 |
100.00%
|
66.67%
|
| Primer 10 | Lactiplantibacillus | TTGGATGCTAAGGGTCGGC | TGTCGCAACGTGGTTGGA | 59.78 | 59.81 | 257 | 140 |
100.00%
|
33.33%
|