| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Ligilactobacillus | TGTTCGAAGCAGGCGACA | TTGCCACCCATACGTCCTG | 59.58 | 59.70 | 196 | 137 |
100.00%
|
33.33%
|
| Primer 2 | Ligilactobacillus | TGTTCGAAGCAGGCGACA | TTGCCACCCATACGTCCTG | 59.58 | 59.70 | 196 | 127 |
100.00%
|
33.33%
|
| Primer 3 | Ligilactobacillus | AGGCTGTTGATGCTGCTCG | ACGCCACGTAAAGCAGCA | 60.74 | 60.28 | 292 | 113 |
100.00%
|
33.33%
|
| Primer 4 | Ligilactobacillus | TGTTCGAAGCAGGCGACA | TCGTTTGCCACCCATACGT | 59.58 | 59.63 | 200 | 112 |
100.00%
|
33.33%
|
| Primer 5 | Ligilactobacillus | GCGTCAAGAAGGAAGCGGA | AATCCGACGCTTGACCCAG | 60.37 | 60.08 | 176 | 110 |
100.00%
|
33.33%
|
| Primer 6 | Ligilactobacillus | AAGCGTCGTGGTGAAGGT | GGCGACGTTCTGGAATACG | 59.18 | 58.71 | 199 | 106 |
100.00%
|
33.33%
|
| Primer 7 | Ligilactobacillus | GCGTCAAGAAGGAAGCGGA | AATCCGACGCTTGACCCA | 60.37 | 58.93 | 176 | 105 |
100.00%
|
33.33%
|
| Primer 8 | Ligilactobacillus | GCGTCAAGAAGGAAGCGGA | GAATCCGACGCTTGACCCA | 60.37 | 60.08 | 177 | 105 |
100.00%
|
33.33%
|
| Primer 9 | Ligilactobacillus | AGGCTGTTGATGCTGCTCG | ACGCCACGTAAAGCAGCA | 60.74 | 60.28 | 292 | 104 |
100.00%
|
33.33%
|
| Primer 10 | Ligilactobacillus | TGTTCGAAGCAGGCGACA | TCGTTTGCCACCCATACGT | 59.58 | 59.63 | 200 | 102 |
100.00%
|
33.33%
|