| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Mesorhizobium | ATCCTGAAGCTGCTGGTGG | AGTTGGCGATCCTGAGCAG | 59.70 | 59.78 | 208 | 121 |
100.00%
|
50.00%
|
| Primer 2 | Mesorhizobium | TGGCGATGTCGATGGCAT | ACATCACCGTCGGGAAGTC | 59.49 | 59.41 | 210 | 109 |
100.00%
|
50.00%
|
| Primer 3 | Mesorhizobium | TGGCGATGTCGATGGCAT | TCGAAGTGAGGGCAAGGTC | 59.49 | 59.33 | 188 | 109 |
100.00%
|
50.00%
|
| Primer 4 | Mesorhizobium | AGACCTTCGCCAATGCCTG | ATTGGGGAAGCTGCGCAT | 60.38 | 60.04 | 161 | 109 |
100.00%
|
50.00%
|
| Primer 5 | Mesorhizobium | TTCGTGCCTCGGTCAAAGG | TTGTCGCGACGATGGCAT | 60.30 | 60.13 | 159 | 109 |
100.00%
|
50.00%
|
| Primer 6 | Mesorhizobium | TCACGTCGCTGACCTTGTC | TGTTGGTGGTGCCGTTGT | 60.01 | 60.04 | 249 | 108 |
100.00%
|
50.00%
|
| Primer 7 | Mesorhizobium | TCACGTCGCTGACCTTGT | TGTTGGTGGTGCCGTTGT | 58.87 | 60.04 | 249 | 108 |
100.00%
|
50.00%
|
| Primer 8 | Mesorhizobium | TGCTCGATGCGATCCTGAG | TTGTCGCGACGATGGCAT | 59.64 | 60.13 | 216 | 108 |
100.00%
|
50.00%
|
| Primer 9 | Mesorhizobium | AGCCAGACCTTCGCCAATG | ATTGGGGAAGCTGCGCAT | 60.38 | 60.04 | 165 | 105 |
100.00%
|
50.00%
|
| Primer 10 | Mesorhizobium | TGGCGATGTCGATGGCAT | CACATCACCGTCGGGAAGT | 59.49 | 59.71 | 211 | 103 |
100.00%
|
50.00%
|