| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methylosinus | ACATGGCGTTGCTGTTGC | GCGAAACAGGAACAGCAGC | 59.66 | 60.08 | 269 | 93 |
100.00%
|
100.00%
|
| Primer 2 | Methylosinus | ACATGGCGTTGCTGTTGC | GGCGAAACAGGAACAGCAG | 59.66 | 59.42 | 270 | 93 |
100.00%
|
100.00%
|
| Primer 3 | Methylosinus | ACGGAAGGGCAAACGCAT | TGCCGTCGAAAACCGTGT | 60.28 | 60.20 | 208 | 83 |
100.00%
|
100.00%
|
| Primer 4 | Methylosinus | TCGCTCATGCATGCCGAT | CCGAGCCGAACAAATGGAC | 59.89 | 59.21 | 156 | 76 |
100.00%
|
100.00%
|
| Primer 5 | Methylosinus | ACCAAAACGCTGCGCATC | GCGAACGCGACTTCCAATC | 59.74 | 59.94 | 265 | 76 |
100.00%
|
100.00%
|
| Primer 6 | Methylosinus | ACCAAAACGCTGCGCATC | ATCGAGCCGAGACGTGAAC | 59.74 | 59.86 | 195 | 71 |
100.00%
|
100.00%
|
| Primer 7 | Methylosinus | ACACGTTTCCGCACCCTT | ACGGTGATCTCGCCATTGC | 59.81 | 60.82 | 242 | 70 |
100.00%
|
100.00%
|
| Primer 8 | Methylosinus | ACACGTTTCCGCACCCTT | TTGCTCTCGAACTCCTCCG | 59.81 | 59.12 | 194 | 70 |
100.00%
|
100.00%
|
| Primer 9 | Methylosinus | ACACGTTTCCGCACCCTT | CGACCACCTTCTCGACCTT | 59.81 | 59.03 | 220 | 70 |
100.00%
|
100.00%
|
| Primer 10 | Methylosinus | ACACGTTTCCGCACCCTT | CACGGTGATCTCGCCATTG | 59.81 | 58.99 | 243 | 70 |
100.00%
|
100.00%
|