| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methylotenera | GGCACGAAACATCACCAGC | ACTTGGCCTGGGTTAGCTG | 59.79 | 59.62 | 250 | 176 |
100.00%
|
100.00%
|
| Primer 2 | Methylotenera | AACGCAGCGCATTTCAGC | ACCGTCACTTGCAGCACT | 60.13 | 59.49 | 240 | 138 |
100.00%
|
50.00%
|
| Primer 3 | Methylotenera | TAGTCACCGCAGCAGATGG | TTTTCACGGTCGCCAACC | 59.48 | 58.58 | 193 | 138 |
100.00%
|
50.00%
|
| Primer 4 | Methylotenera | TGAAAACCAAGAGCGGTGC | CGCGTTTGACGTCTGTTGG | 58.97 | 60.08 | 162 | 135 |
100.00%
|
50.00%
|
| Primer 5 | Methylotenera | TCGCGTGATGACTCGTCAC | AGCAGCAGCCAAACGGAA | 60.15 | 60.20 | 217 | 128 |
100.00%
|
100.00%
|
| Primer 6 | Methylotenera | ATTGGCGTGCACACTGGT | AGCAGCACCAGCAACGAT | 60.20 | 59.97 | 177 | 128 |
100.00%
|
100.00%
|
| Primer 7 | Methylotenera | ACGTCACGCACTCGGAAT | TGCGGTGCGAAGTTCTCT | 59.35 | 59.27 | 271 | 127 |
100.00%
|
50.00%
|
| Primer 8 | Methylotenera | TGGTTGGTTTGTGGCCAGT | AGCCATTGTGCGCTCCTT | 60.00 | 59.97 | 267 | 125 |
100.00%
|
50.00%
|
| Primer 9 | Methylotenera | TACTAGCAGCCATCGTGCG | CCATTGGTGCATTGTCGCC | 59.93 | 60.15 | 296 | 124 |
100.00%
|
50.00%
|
| Primer 10 | Methylotenera | TGAAAACCAAGAGCGGTGC | CGAACGCGTTTGACGTCTG | 58.97 | 60.16 | 166 | 124 |
100.00%
|
50.00%
|