| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methylovorus | GCCTGCGCTACTTCAATGC | AATTTGCGCAAGGCCAGC | 59.94 | 60.05 | 226 | 157 |
100.00%
|
100.00%
|
| Primer 2 | Methylovorus | ATTTGCCAGTGCGCTTGC | TACCGGTTTTGGCTCCCAC | 60.05 | 59.93 | 284 | 157 |
100.00%
|
100.00%
|
| Primer 3 | Methylovorus | TCGCTGCACGTGTTTTGC | ACGACTGAGATTGGGCTGC | 59.98 | 60.08 | 243 | 157 |
100.00%
|
100.00%
|
| Primer 4 | Methylovorus | TCGCTGCACGTGTTTTGC | TGGGCTGCAAACCTCTTCC | 59.98 | 60.23 | 232 | 157 |
100.00%
|
100.00%
|
| Primer 5 | Methylovorus | TGGTGCGTAGTGTGGTGT | ACTTCAAGGCCAGCGCAT | 58.78 | 59.97 | 285 | 148 |
100.00%
|
100.00%
|
| Primer 6 | Methylovorus | GTGGTGCGTAGTGTGGTGT | ACTTCAAGGCCAGCGCAT | 60.23 | 59.97 | 286 | 148 |
100.00%
|
100.00%
|
| Primer 7 | Methylovorus | TGCTCTGCTTCAGCCTGT | TTGCCGTCACTGCCACAT | 58.85 | 59.89 | 177 | 148 |
100.00%
|
100.00%
|
| Primer 8 | Methylovorus | GTGCTCTGCTTCAGCCTGT | TTGCCGTCACTGCCACAT | 60.30 | 59.89 | 178 | 148 |
100.00%
|
100.00%
|
| Primer 9 | Methylovorus | TTCTGAAGGTCAGCGCTGG | ATCCAGCACGCCATAGAGC | 60.00 | 59.93 | 256 | 143 |
100.00%
|
100.00%
|
| Primer 10 | Methylovorus | ATGGAAAGCCCTGCGCAT | ATCTGTCCACCACTTCGGC | 60.04 | 59.70 | 211 | 143 |
100.00%
|
100.00%
|