| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Mobiluncus | TGCGCAAACTGACGGGAT | CGACACGTTCCGCAAAGTC | 59.97 | 59.80 | 257 | 141 |
100.00%
|
40.00%
|
| Primer 2 | Mobiluncus | TGCGCAAACTGACGGGAT | TCGACACGTTCCGCAAAGT | 59.97 | 60.23 | 258 | 141 |
100.00%
|
40.00%
|
| Primer 3 | Mobiluncus | TGACCGGAAAGCGCTTGT | AAAGGTCGTGTAGGCACCC | 59.89 | 59.63 | 191 | 140 |
100.00%
|
40.00%
|
| Primer 4 | Mobiluncus | ACTGCGCAATGTGCTGGA | TGTCACGCAAGGAATCCCC | 60.28 | 60.00 | 257 | 140 |
100.00%
|
20.00%
|
| Primer 5 | Mobiluncus | TCAACACGGCACTGCACT | TCGAGTGCCAAGCCGTATC | 59.81 | 59.86 | 256 | 138 |
100.00%
|
40.00%
|
| Primer 6 | Mobiluncus | TCAACACGGCACTGCACT | TGGCAGCAATCCGATCGAG | 59.81 | 60.23 | 270 | 138 |
100.00%
|
40.00%
|
| Primer 7 | Mobiluncus | CCCACAATTCACTGCTGCC | TGAGCACGTTCGGCAACT | 59.41 | 59.89 | 208 | 138 |
100.00%
|
20.00%
|
| Primer 8 | Mobiluncus | GCCTTCTTTCGTGGGCATC | TGAGCACGTTCGGCAACT | 59.20 | 59.89 | 192 | 138 |
100.00%
|
20.00%
|
| Primer 9 | Mobiluncus | AGGAATGCACGGCAAGGA | ATTCCGCAATCCGCAGCA | 59.24 | 60.44 | 212 | 138 |
100.00%
|
40.00%
|
| Primer 10 | Mobiluncus | AAGGATGGCGATGGTCTGC | ATTCCGCAATCCGCAGCA | 60.15 | 60.44 | 199 | 138 |
100.00%
|
40.00%
|