| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Mycobacterium | ATGAGCACGACGTTCGCT | ATGCGGAAGAAGTTGGCCA | 59.74 | 59.93 | 209 | 103 |
90.91%
|
111.11%
|
| Primer 2 | Mycobacterium | TCTCGACAACGTCAAGCCC | AGGATCTCGTTTGCCAGCC | 60.01 | 60.08 | 180 | 76 |
100.00%
|
55.56%
|
| Primer 3 | Mycobacterium | ATTCTTGACCCCACGCTCG | ACGACGTAGATGCCTTCGG | 60.08 | 59.57 | 161 | 70 |
100.00%
|
55.56%
|
| Primer 4 | Mycobacterium | TCTCGACAACGTCAAGCCC | AGGATCTCGTTTGCCAGGC | 60.01 | 60.08 | 180 | 65 |
90.91%
|
111.11%
|
| Primer 5 | Mycobacterium | ATGAGCACGACGTTCGCT | GCGGAAGAAGTTGGCAAGG | 59.74 | 59.42 | 207 | 65 |
90.91%
|
111.11%
|
| Primer 6 | Mycobacterium | AGGGGAAGAAATCGCTCGC | AGGATCTCGTTTGCCAGGC | 60.15 | 60.08 | 277 | 63 |
90.91%
|
111.11%
|
| Primer 7 | Mycobacterium | CCACCGACATCACCAACGA | ACATGGTGCCGTTGACGT | 60.01 | 59.89 | 180 | 63 |
100.00%
|
55.56%
|
| Primer 8 | Mycobacterium | GGGTGAAGCTGACGAGTCA | AGCCTGCTTGCGGTTCTT | 59.33 | 59.89 | 179 | 62 |
100.00%
|
55.56%
|
| Primer 9 | Mycobacterium | ATCCAGGTGCTGCTGATCG | CCAGGTCCTTGAACCGCAT | 59.85 | 60.00 | 265 | 61 |
90.91%
|
111.11%
|
| Primer 10 | Mycobacterium | CGAACATGGATCCCGAGCA | GGCGTCATGTCGTAGAGCA | 59.86 | 59.86 | 181 | 59 |
90.91%
|
111.11%
|