| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Paeniclostridium | ATGCAGGCCACGCTATAGC | CCAAGACATGTGACCTCCTGG | 60.30 | 60.34 | 185 | 165 |
100.00%
|
100.00%
|
| Primer 2 | Paeniclostridium | ATGCAGGCCACGCTATAGC | TCCCTTCCAAGACATGTGACC | 60.30 | 59.65 | 191 | 165 |
100.00%
|
100.00%
|
| Primer 3 | Paeniclostridium | ATGTCGGAACTGGTGCTGG | TAGGTAGCCCAGCAACTGC | 60.00 | 59.40 | 237 | 157 |
100.00%
|
100.00%
|
| Primer 4 | Paeniclostridium | TGATGTCGGAACTGGTGCTG | TAGGTAGCCCAGCAACTGC | 60.32 | 59.40 | 239 | 146 |
100.00%
|
100.00%
|
| Primer 5 | Paeniclostridium | GCTCTGCTAAAGATCAAGGGC | TCATTGAGCTTCGCCTACCTC | 59.05 | 59.86 | 287 | 143 |
100.00%
|
100.00%
|
| Primer 6 | Paeniclostridium | GCTCTGCTAAAGATCAAGGGC | CATTGAGCTTCGCCTACCTC | 59.05 | 58.42 | 286 | 143 |
100.00%
|
100.00%
|
| Primer 7 | Paeniclostridium | ATGCAGGCCACGCTATAGC | ACATGTGACCTCCTGGACATC | 60.30 | 59.44 | 180 | 140 |
100.00%
|
100.00%
|
| Primer 8 | Paeniclostridium | ATGCAGGCCACGCTATAGC | GACATGTGACCTCCTGGACAT | 60.30 | 59.44 | 181 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Paeniclostridium | GACACTATGGGTCCAACTGCA | AGTGCCCATGCTAGTGTAGT | 60.00 | 58.43 | 152 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Paeniclostridium | AGTGAAGGGTTAGCACCTGT | ACCGCTTTCAAAGTTGGCA | 58.56 | 58.50 | 233 | 138 |
100.00%
|
100.00%
|