| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Peptoniphilus | TGAGAGCAGACAAGGAAGTCC | TAGGTGGGCTGTCAGGACA | 59.38 | 59.84 | 173 | 115 |
100.00%
|
46.67%
|
| Primer 2 | Peptoniphilus | AGCAGACAAGGAAGTCCAACT | TAGGTGGGCTGTCAGGACA | 59.23 | 59.84 | 169 | 115 |
100.00%
|
46.67%
|
| Primer 3 | Peptoniphilus | GAGAGCAGACAAGGAAGTCCA | TAGGTGGGCTGTCAGGACA | 59.38 | 59.84 | 172 | 113 |
100.00%
|
46.67%
|
| Primer 4 | Peptoniphilus | AGAGCAGACAAGGAAGTCCA | TAGGTGGGCTGTCAGGACA | 58.28 | 59.84 | 171 | 113 |
100.00%
|
46.67%
|
| Primer 5 | Peptoniphilus | AAAAGAGGCGACCTTCACG | TTTCTGCACCGTCTCCACG | 58.09 | 60.30 | 163 | 110 |
100.00%
|
46.67%
|
| Primer 6 | Peptoniphilus | TTGGCAGAAGTGGTTCCGG | TTGTGGCACAAAACCTATGGT | 60.23 | 58.33 | 161 | 110 |
100.00%
|
40.00%
|
| Primer 7 | Peptoniphilus | ATTGGCAGAAGTGGTTCCGG | TTGTGGCACAAAACCTATGGT | 60.61 | 58.33 | 162 | 110 |
100.00%
|
40.00%
|
| Primer 8 | Peptoniphilus | GCGTGGTAATGAGCTTGCA | GCACCTTTACCGGAACCCA | 58.83 | 59.93 | 201 | 102 |
100.00%
|
46.67%
|
| Primer 9 | Peptoniphilus | TGCGTGGTAATGAGCTTGC | GCACCTTTACCGGAACCCA | 58.83 | 59.93 | 202 | 102 |
100.00%
|
46.67%
|
| Primer 10 | Peptoniphilus | AGGCAGAATGAAGGGCAAGG | GCACCTTTACCGGAACCCA | 60.32 | 59.93 | 225 | 102 |
90.91%
|
66.67%
|