| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Phocaeicola | ACGACTGTTGCGAAGGCT | CCGGACGATCTGCAACCTT | 59.58 | 60.08 | 169 | 103 |
100.00%
|
47.06%
|
| Primer 2 | Phocaeicola | TTCAGCGTCGTGTTCCGA | ACGGCTTCGATTGCAGCT | 59.28 | 60.05 | 253 | 98 |
100.00%
|
47.06%
|
| Primer 3 | Phocaeicola | TCTTCAGCGTCGTGTTCCG | ACGGCTTCGATTGCAGCT | 60.37 | 60.05 | 255 | 95 |
100.00%
|
47.06%
|
| Primer 4 | Phocaeicola | GCCTCTTCAGCGTCGTCTT | ACGGCTTCGATTGCAGCT | 60.08 | 60.05 | 258 | 95 |
100.00%
|
35.29%
|
| Primer 5 | Phocaeicola | ATGCCTCTTCAGCGTCGT | ACGGCTTCGATTGCAGCT | 59.03 | 60.05 | 260 | 92 |
100.00%
|
35.29%
|
| Primer 6 | Phocaeicola | ATGCCTCTTCAGCGTCGTC | ACGGCTTCGATTGCAGCT | 60.15 | 60.05 | 260 | 88 |
100.00%
|
35.29%
|
| Primer 7 | Phocaeicola | TCTTCAGCGTCGTCTTCCG | ACGGCTTCGATTGCAGCT | 59.79 | 60.05 | 255 | 88 |
100.00%
|
35.29%
|
| Primer 8 | Phocaeicola | ACGCAGCATCGCCATCAA | TGCGTTGCGGACACTTACT | 60.44 | 59.93 | 208 | 86 |
100.00%
|
47.06%
|
| Primer 9 | Phocaeicola | ACGCAGCATCGCCATCAA | GTGCGTTGCGGACACTTAC | 60.44 | 59.80 | 209 | 86 |
100.00%
|
47.06%
|
| Primer 10 | Phocaeicola | ATGCCTCTTCAGCGTCGT | ACGGCTTCGATTGCAGCT | 59.03 | 60.05 | 260 | 79 |
100.00%
|
35.29%
|