| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Polynucleobacter | TGCTATCCCGAATCGCGAG | TGCTGCCATCGAGCTTGT | 59.71 | 59.65 | 182 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Polynucleobacter | GCTATCCCGAATCGCGAGT | TGCTGCCATCGAGCTTGT | 59.71 | 59.65 | 181 | 143 |
100.00%
|
100.00%
|
| Primer 3 | Polynucleobacter | ACCGGTGATGCATTGCCA | CTTCAAACACTGCCTGGGC | 59.96 | 59.34 | 152 | 135 |
100.00%
|
100.00%
|
| Primer 4 | Polynucleobacter | ACCGGTGATGCATTGCCA | TTCAAACACTGCCTGGGC | 59.96 | 58.15 | 151 | 135 |
100.00%
|
100.00%
|
| Primer 5 | Polynucleobacter | ACTGGGAAGAAGCCGATGG | AATTTCGCCACGTGCACG | 59.40 | 59.75 | 210 | 132 |
100.00%
|
100.00%
|
| Primer 6 | Polynucleobacter | AGTCACTGGGAAGAAGCCG | AATTTCGCCACGTGCACG | 59.33 | 59.75 | 214 | 132 |
100.00%
|
100.00%
|
| Primer 7 | Polynucleobacter | AAGTCACTGGGAAGAAGCCG | AATTTCGCCACGTGCACG | 59.96 | 59.75 | 215 | 132 |
100.00%
|
100.00%
|
| Primer 8 | Polynucleobacter | ACCGGTGATGCATTGCCA | TTCAAACACTGCCTGGGCT | 59.96 | 59.77 | 151 | 128 |
100.00%
|
100.00%
|
| Primer 9 | Polynucleobacter | GCCCACCTGCTTCCAGAAT | TGCTGCCATCGAGCTTGT | 60.00 | 59.65 | 228 | 128 |
100.00%
|
100.00%
|
| Primer 10 | Polynucleobacter | CTGGGAAGAAGCCGATGGT | AATTTCGCCACGTGCACG | 59.40 | 59.75 | 209 | 128 |
100.00%
|
100.00%
|