| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Pseudomonas | AAGATCTGCTGCGCTGCA | TGGCAGGCAAAGAACCACA | 60.05 | 60.08 | 181 | 127 |
100.00%
|
47.06%
|
| Primer 2 | Pseudomonas | AAGATCTGCTGCGCTGCA | TGCCAGCCGAGGAAGAAA | 60.05 | 58.84 | 262 | 127 |
100.00%
|
26.47%
|
| Primer 3 | Pseudomonas | ACGGCATCATCGGCATGA | TCCGTTCGATGCTGTAGCC | 59.49 | 59.86 | 183 | 126 |
90.91%
|
29.41%
|
| Primer 4 | Pseudomonas | ACGGCATCATCGGCATGA | CCGTTCGATGCTGTAGCCA | 59.49 | 60.15 | 182 | 126 |
91.67%
|
32.35%
|
| Primer 5 | Pseudomonas | AAGATCTGCTGCGCTGCA | AGTGGCAGGCAAAGAACCA | 60.05 | 59.77 | 183 | 125 |
100.00%
|
47.06%
|
| Primer 6 | Pseudomonas | AACTGCGACGTCTGCGAA | CGGTACTTGGCCATCAGCT | 59.97 | 59.78 | 200 | 118 |
100.00%
|
35.29%
|
| Primer 7 | Pseudomonas | AACAACATCGCCAACGCC | TTGAGCAGGGTGAAGCTGG | 59.36 | 59.93 | 284 | 118 |
92.86%
|
38.24%
|
| Primer 8 | Pseudomonas | AACAACATCGCCAACGCC | GTGAAGCTGGCCTGGAAGT | 59.36 | 59.93 | 275 | 118 |
93.33%
|
41.18%
|
| Primer 9 | Pseudomonas | CAGGTGAACGTGCACAAGC | ACAGCTTGCCGTTGAGGT | 60.01 | 59.49 | 280 | 118 |
93.33%
|
41.18%
|
| Primer 10 | Pseudomonas | AGCGAAGACCGTAGCGAAC | ACAGCTTGCCGTTGAGGT | 60.15 | 59.49 | 241 | 118 |
100.00%
|
32.35%
|