| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Rhodobacter | GCCAGTTTCTTGCCCTTGC | TTCGCAGAACGGACAGACC | 60.01 | 60.01 | 236 | 158 |
100.00%
|
50.00%
|
| Primer 2 | Rhodobacter | ACCTCGCTGGAAAGTCAGG | AACTCGCCTCGCCATTGT | 59.33 | 59.65 | 241 | 138 |
100.00%
|
50.00%
|
| Primer 3 | Rhodobacter | TGGCAAACCCGATCCAGT | TGCCTTGATCCCGAGGTTG | 58.83 | 59.70 | 170 | 133 |
100.00%
|
50.00%
|
| Primer 4 | Rhodobacter | TCGATGTGACCTCGCTGGA | AACTCGCCTCGCCATTGT | 60.68 | 59.65 | 249 | 130 |
100.00%
|
50.00%
|
| Primer 5 | Rhodobacter | ACGACAAGCTGATCGGCA | TCGACATGAACGCTGGCA | 59.34 | 59.66 | 280 | 128 |
100.00%
|
50.00%
|
| Primer 6 | Rhodobacter | TGGCTGGATGTGGCTCATG | ACGACGGAAAACAGCGCA | 60.08 | 60.59 | 162 | 128 |
100.00%
|
50.00%
|
| Primer 7 | Rhodobacter | CTCAAATCCGCAAGCCTGC | ACGACGGAAAACAGCGCA | 59.86 | 60.59 | 245 | 128 |
100.00%
|
50.00%
|
| Primer 8 | Rhodobacter | GCTCAAATCCGCAAGCCTG | ACGACGGAAAACAGCGCA | 59.86 | 60.59 | 246 | 128 |
100.00%
|
50.00%
|
| Primer 9 | Rhodobacter | TCAAATCCGCAAGCCTGC | ACGACGGAAAACAGCGCA | 58.72 | 60.59 | 244 | 128 |
100.00%
|
50.00%
|
| Primer 10 | Rhodobacter | CAACGACGCGATGGATGTG | TGCCCATCTTGCCATGCA | 59.66 | 59.96 | 230 | 128 |
100.00%
|
50.00%
|