| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Schaalia | AAGACCGTCATGAGCCAGG | TGAGCTCGTTCGCGTTGT | 59.40 | 59.97 | 289 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Schaalia | GCGCTTCAAGACCGTCATG | TGAGCTCGTTCGCGTTGT | 59.58 | 59.97 | 296 | 145 |
100.00%
|
100.00%
|
| Primer 3 | Schaalia | GCGCAACGATCGACAACA | AGCCTTGATGTCCTCGTGG | 59.15 | 59.40 | 248 | 137 |
100.00%
|
100.00%
|
| Primer 4 | Schaalia | GCGCAACGATCGACAACA | TGTCCTCGTGGTGTCGGAT | 59.15 | 60.61 | 240 | 137 |
100.00%
|
100.00%
|
| Primer 5 | Schaalia | GCGCAACGATCGACAACA | ATGTCCTCGTGGTGTCGGA | 59.15 | 60.61 | 241 | 137 |
100.00%
|
100.00%
|
| Primer 6 | Schaalia | GGCACGTTTGGTCAACACG | AGCTGCGCAAACGAGTGA | 60.30 | 60.28 | 186 | 132 |
100.00%
|
50.00%
|
| Primer 7 | Schaalia | GCAGGCACGTTTGGTCAAC | AGCTGCGCAAACGAGTGA | 60.30 | 60.28 | 189 | 132 |
100.00%
|
50.00%
|
| Primer 8 | Schaalia | TCGTTGACCTGCTGGAACG | AGCTGCGCAAACGAGTGA | 60.30 | 60.28 | 166 | 132 |
100.00%
|
50.00%
|
| Primer 9 | Schaalia | TCGTACGTCGAGATGCACC | AGCTGCGCAAACGAGTGA | 59.57 | 60.28 | 218 | 132 |
100.00%
|
50.00%
|
| Primer 10 | Schaalia | GCGCAACGATCGACAACA | TCCTCGTGGTGTCGGATGA | 59.15 | 60.30 | 238 | 132 |
100.00%
|
100.00%
|