| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Slackia | GCTGCTCATCATCGACGGA | TGCCTGCGGTGGAAAACA | 59.93 | 60.12 | 251 | 137 |
100.00%
|
50.00%
|
| Primer 2 | Slackia | CCGCAAGCTGCTCATCATC | TGCCTGCGGTGGAAAACA | 59.35 | 60.12 | 257 | 137 |
100.00%
|
50.00%
|
| Primer 3 | Slackia | TCGATGATCGCCAAGGCTG | ACCAGGAAGCCTTGGTCAC | 60.23 | 59.54 | 172 | 136 |
100.00%
|
50.00%
|
| Primer 4 | Slackia | AGCTGCTCATCATCGACGG | TGCCTGCGGTGGAAAACA | 59.93 | 60.12 | 252 | 130 |
100.00%
|
50.00%
|
| Primer 5 | Slackia | GGGAACGCTACCTGCATCT | TGCCTGCGGTGGAAAACA | 59.48 | 60.12 | 213 | 127 |
100.00%
|
50.00%
|
| Primer 6 | Slackia | ACCATCGTCGTGAAGGTCG | ACGCCAGCGCTTCATCTT | 59.79 | 60.05 | 165 | 124 |
100.00%
|
50.00%
|
| Primer 7 | Slackia | ACGGATACAACGTCCTGCG | TGCCTGCGGTGGAAAACA | 60.15 | 60.12 | 237 | 124 |
100.00%
|
50.00%
|
| Primer 8 | Slackia | CGCAAGCTGCTCATCATCG | TGCCTGCGGTGGAAAACA | 59.72 | 60.12 | 256 | 124 |
100.00%
|
50.00%
|
| Primer 9 | Slackia | ACGTCATCGCTCGTTGCA | ACTTCTTGCGCTCGACCA | 60.05 | 59.27 | 159 | 124 |
100.00%
|
50.00%
|
| Primer 10 | Slackia | ACGCGTGCGGAATTCGAT | CGCTTCGGCAATACAGCAC | 60.51 | 59.94 | 178 | 119 |
100.00%
|
50.00%
|