| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Sporanaerobacter | TGAAGGACTGCTGGACTTAGC | GCTTTTGCCCTGGTAGAAGC | 59.72 | 59.47 | 219 | 151 |
100.00%
|
100.00%
|
| Primer 2 | Sporanaerobacter | TGAAGGACTGCTGGACTTAGC | GACTCTTGCTTTTGCCCTGG | 59.72 | 59.40 | 226 | 151 |
100.00%
|
100.00%
|
| Primer 3 | Sporanaerobacter | TGAAGGACTGCTGGACTTAGC | TGCCCTGGTAGAAGCCATTC | 59.72 | 59.74 | 214 | 148 |
100.00%
|
100.00%
|
| Primer 4 | Sporanaerobacter | TGGGTTTTGACGGGTTTAAGG | GCAACCAATCCTGAAGCACC | 58.69 | 59.76 | 265 | 132 |
100.00%
|
100.00%
|
| Primer 5 | Sporanaerobacter | GCCAACTGTAGGAGCCTCTG | TATGAGCCCACCATGCCAC | 60.11 | 59.77 | 218 | 127 |
100.00%
|
100.00%
|
| Primer 6 | Sporanaerobacter | GCCGAAAGCAATCCTTCACC | ACTGCACCTATACCTCCTGC | 59.83 | 58.88 | 170 | 126 |
100.00%
|
100.00%
|
| Primer 7 | Sporanaerobacter | GGAGCCTCTGGAGCTATTGC | TATGAGCCCACCATGCCAC | 60.25 | 59.77 | 208 | 126 |
100.00%
|
100.00%
|
| Primer 8 | Sporanaerobacter | TGAAGGACTGCTGGACTTAGC | TTGCCCTGGTAGAAGCCAT | 59.72 | 58.60 | 215 | 124 |
100.00%
|
100.00%
|
| Primer 9 | Sporanaerobacter | TGGGTTTTGACGGGTTTAAGG | AGCAACCAATCCTGAAGCACC | 58.69 | 61.16 | 266 | 124 |
100.00%
|
100.00%
|
| Primer 10 | Sporanaerobacter | TGGGGTTCAGTAGCACCAG | GTCCATCTTTCCGCATGTGG | 58.93 | 59.27 | 227 | 123 |
100.00%
|
100.00%
|