| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Yersinia | TGCACCAAACGGTCTGCA | TTCGCCTTCAGCCGAGATC | 60.12 | 59.86 | 178 | 138 |
100.00%
|
35.71%
|
| Primer 2 | Yersinia | TGCACCAAACGGTCTGCA | CTTCGCCTTCAGCCGAGAT | 60.12 | 59.86 | 179 | 138 |
90.91%
|
35.71%
|
| Primer 3 | Yersinia | TGCACCAAACGGTCTGCA | ATCTTCGCCTTCAGCCGAG | 60.12 | 59.86 | 181 | 138 |
100.00%
|
35.71%
|
| Primer 4 | Yersinia | TGCACCAAACGGTCTGCA | TTCGCCTTCAGCCGAGAT | 60.12 | 58.70 | 178 | 138 |
100.00%
|
32.14%
|
| Primer 5 | Yersinia | CCTGATCAACCCAGCAGCA | ACCGCTGCCTGTAAAGCA | 60.00 | 59.57 | 207 | 138 |
100.00%
|
35.71%
|
| Primer 6 | Yersinia | TCCTGATCAACCCAGCAGC | ACCGCTGCCTGTAAAGCA | 59.70 | 59.57 | 208 | 138 |
100.00%
|
39.29%
|
| Primer 7 | Yersinia | CGCTTTGGCAACCGCAAA | GAGTAATCGCGAGGTGGCA | 60.28 | 59.86 | 293 | 137 |
100.00%
|
28.57%
|
| Primer 8 | Yersinia | CGCTTTGGCAACCGCAAA | CGCGAGGTGGCATTTTCAC | 60.28 | 60.15 | 286 | 137 |
100.00%
|
28.57%
|
| Primer 9 | Yersinia | ACGCAGTGCAACAGTTGG | TTTGGTACGCTGTGGCCA | 58.89 | 59.49 | 188 | 135 |
100.00%
|
25.00%
|
| Primer 10 | Yersinia | TGCACCAAACGGTCTGCA | TCATCTTCGCCTTCAGCCG | 60.12 | 60.15 | 183 | 135 |
90.91%
|
35.71%
|