| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Acidithiobacillus ferrooxidans | CGCCTTCAACGTCTTTGGC | GCTTCCTGCAGTTCGCTCT | 60.08 | 60.38 | 177 | 83 |
100.00%
|
100.00%
|
| Primer 302 | Acidithiobacillus ferrooxidans | ATCCGCAACACTGGCACT | TTGTTGTCCGGAAGCCTCC | 59.57 | 59.93 | 257 | 82 |
100.00%
|
100.00%
|
| Primer 303 | Acidithiobacillus ferrooxidans | AATGTCAGCGGAGTGGCA | CAACACGCTCACACGCATC | 59.25 | 60.15 | 208 | 82 |
100.00%
|
100.00%
|
| Primer 304 | Acidithiobacillus ferrooxidans | AATGTCAGCGGAGTGGCA | AACACGCTCACACGCATC | 59.25 | 58.76 | 207 | 82 |
100.00%
|
100.00%
|
| Primer 305 | Acidithiobacillus ferrooxidans | CGCATCGGCATCTACGACT | GTTTGTGCACCGTCACCTG | 60.01 | 59.64 | 256 | 82 |
100.00%
|
100.00%
|
| Primer 306 | Acidithiobacillus ferrooxidans | CAGTCCCGAGGCGAAACAT | ATCAAGTTCCTGCCAGGCC | 60.08 | 60.00 | 174 | 82 |
100.00%
|
100.00%
|
| Primer 307 | Acidithiobacillus ferrooxidans | AGTCCCGAGGCGAAACATC | ATCAAGTTCCTGCCAGGCC | 59.78 | 60.00 | 173 | 82 |
100.00%
|
100.00%
|
| Primer 308 | Acidithiobacillus ferrooxidans | CAGACCTGCAATCCGGTGT | TGATATCCGCAGCCAGCA | 60.00 | 58.77 | 219 | 82 |
100.00%
|
100.00%
|
| Primer 309 | Acidithiobacillus ferrooxidans | AATGGTGCGGACCTTGCA | GTCGCTTCAAGCTTGCCAG | 59.88 | 59.79 | 178 | 82 |
100.00%
|
100.00%
|
| Primer 310 | Acidithiobacillus ferrooxidans | AGGGTCTGGGTATCCGCAT | TGAAGTCCAGTTCGGTGCC | 60.07 | 59.93 | 174 | 82 |
100.00%
|
100.00%
|