| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Acidithiobacillus ferrooxidans | ACGCAATCCGGTCATGCA | GATGGCATGAACCAGCAGC | 60.05 | 59.56 | 154 | 127 |
100.00%
|
100.00%
|
| Primer 42 | Acidithiobacillus ferrooxidans | ACGCAATCCGGTCATGCA | ATGGCATGAACCAGCAGC | 60.05 | 58.39 | 153 | 127 |
100.00%
|
100.00%
|
| Primer 43 | Acidithiobacillus ferrooxidans | TGCTGCTGCGTCTGGAAA | CAGCGCTTTGAGTTGCAGG | 59.89 | 60.08 | 251 | 126 |
100.00%
|
100.00%
|
| Primer 44 | Acidithiobacillus ferrooxidans | TGCTGCTGCGTCTGGAAA | GCGCTTTGAGTTGCAGGTC | 59.89 | 60.08 | 249 | 126 |
100.00%
|
100.00%
|
| Primer 45 | Acidithiobacillus ferrooxidans | TTACGGTTTTGCGCTCGC | GATCTCGACATCGACGCCA | 59.45 | 59.64 | 155 | 125 |
100.00%
|
100.00%
|
| Primer 46 | Acidithiobacillus ferrooxidans | TTACGGTTTTGCGCTCGC | TGATCTCGACATCGACGCC | 59.45 | 59.64 | 156 | 125 |
100.00%
|
100.00%
|
| Primer 47 | Acidithiobacillus ferrooxidans | TTACGGTTTTGCGCTCGC | ATCTCGACATCGACGCCAG | 59.45 | 59.64 | 154 | 125 |
100.00%
|
100.00%
|
| Primer 48 | Acidithiobacillus ferrooxidans | TCGTGTTACGGGATGCCAC | ACCATCGTTCCTTGGCAGG | 60.08 | 60.00 | 293 | 125 |
100.00%
|
100.00%
|
| Primer 49 | Acidithiobacillus ferrooxidans | ACGTCTGCTCGCAGTGAT | CGTATGCATTCCCTTGCGC | 59.03 | 60.01 | 151 | 125 |
100.00%
|
100.00%
|
| Primer 50 | Acidithiobacillus ferrooxidans | ATCGAGCATGTGGAGCCTG | ACACCACAAAAGCGCTGC | 59.85 | 59.59 | 193 | 124 |
100.00%
|
100.00%
|