| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Alkalihalobacillus alcalophilus | ATGCGTGGAAGCGATTGC | CGCTTCCCGAACATCTAATGC | 59.21 | 59.74 | 184 | 132 |
100.00%
|
100.00%
|
| Primer 12 | Alkalihalobacillus alcalophilus | TGTTCGTGCACACCGTGT | TGAGCTCAGGCGTTTTGC | 60.13 | 58.66 | 263 | 130 |
100.00%
|
100.00%
|
| Primer 13 | Alkalihalobacillus alcalophilus | GGCAATGGCTCACTTCAAGC | ACCCTCCAGAAACGGCTTG | 60.11 | 59.93 | 216 | 126 |
100.00%
|
100.00%
|
| Primer 14 | Alkalihalobacillus alcalophilus | CCCTGACTCTGATGACCCAG | CAGGGCTATTGTCGGGAGT | 59.17 | 58.79 | 155 | 125 |
100.00%
|
100.00%
|
| Primer 15 | Alkalihalobacillus alcalophilus | AGAATCGGTGCGAGTTGC | TCAACGACGCGAACTTGC | 58.12 | 59.07 | 225 | 123 |
100.00%
|
100.00%
|
| Primer 16 | Alkalihalobacillus alcalophilus | ATCGCAGAGGCATTGGAGG | TCGCCAATCCGATCAATTGC | 59.85 | 59.34 | 263 | 123 |
100.00%
|
100.00%
|
| Primer 17 | Alkalihalobacillus alcalophilus | TGATGCTGGATGTGGCTGG | AGGGAAGCGGAGTTGATGC | 60.08 | 60.08 | 195 | 123 |
100.00%
|
100.00%
|
| Primer 18 | Alkalihalobacillus alcalophilus | GCTTTCAAGAGCTGCCACC | GGATCGGGTACTGTGCCAT | 59.42 | 59.17 | 240 | 122 |
100.00%
|
100.00%
|
| Primer 19 | Alkalihalobacillus alcalophilus | TGCGTGGTTGGAAGCTGT | TTGGCCATCATTCGACCCC | 59.81 | 60.08 | 180 | 122 |
100.00%
|
100.00%
|
| Primer 20 | Alkalihalobacillus alcalophilus | TTGTTCGCTCCCCTGAAGG | TCCACGCTCTCTTGCAAGC | 59.63 | 60.67 | 188 | 121 |
100.00%
|
100.00%
|