| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Alkalihalobacillus clausii | ACATGCGCATGGAACGGT | ACAAGCGTCGGCAACTGA | 60.36 | 59.89 | 225 | 66 |
100.00%
|
100.00%
|
| Primer 522 | Alkalihalobacillus clausii | TCCATTGTCGCATCGGAGC | GCGCAACTGCCCACAAAT | 60.52 | 59.66 | 153 | 66 |
100.00%
|
100.00%
|
| Primer 523 | Alkalihalobacillus clausii | TGTCCATTGTCGCATCGGA | GCGCAACTGCCCACAAAT | 59.41 | 59.66 | 155 | 66 |
100.00%
|
100.00%
|
| Primer 524 | Alkalihalobacillus clausii | AGTGCGTCGCCTTTTCCA | ATTCGGCTCTATCGTCGCC | 59.89 | 59.71 | 236 | 66 |
100.00%
|
100.00%
|
| Primer 525 | Alkalihalobacillus clausii | TTTCGAGGCCTTGTCGCA | TCTTCATGCTGCACGCCA | 59.58 | 59.97 | 160 | 66 |
100.00%
|
100.00%
|
| Primer 526 | Alkalihalobacillus clausii | GTGGAAACGTTGCTTGCCA | AAACCGGTCGTCTGCCAT | 59.56 | 59.25 | 270 | 66 |
100.00%
|
100.00%
|
| Primer 527 | Alkalihalobacillus clausii | ACGTAGCTTGCTTCGCGT | ATGTCCGGTAGCCAAGCAC | 60.05 | 60.08 | 218 | 66 |
100.00%
|
100.00%
|
| Primer 528 | Alkalihalobacillus clausii | AGTGCAAGCAATTGCCCA | ATCGACTTGAGCTGCGTCC | 58.13 | 60.15 | 193 | 66 |
100.00%
|
100.00%
|
| Primer 529 | Alkalihalobacillus clausii | TGCTGGCACTGGCATACA | AACCCAGTTAGAGCAGCCG | 59.24 | 59.70 | 153 | 66 |
100.00%
|
100.00%
|
| Primer 530 | Alkalihalobacillus clausii | ATGCCAACCGGAGCTTGT | TGCGAAATGCCTAGGCGT | 59.56 | 59.73 | 229 | 66 |
100.00%
|
100.00%
|