| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Anaerocolumna aminovalerica | ATGGCAATAGGAGCGGACG | CCAACGCTTCTGCTGCTTC | 59.93 | 59.79 | 268 | 110 |
100.00%
|
100.00%
|
| Primer 302 | Anaerocolumna aminovalerica | TTGGCTATGGCACCGGAAG | ACCAAATCTGCGAGCGTGA | 60.08 | 60.01 | 197 | 110 |
100.00%
|
100.00%
|
| Primer 303 | Anaerocolumna aminovalerica | TCGGCTTGACGGTGTTGA | ATCCGCAGCTGTTCCAGT | 59.18 | 58.93 | 207 | 110 |
100.00%
|
100.00%
|
| Primer 304 | Anaerocolumna aminovalerica | AGATGCAGAATCAGCCTGTGA | CGGGAAACTACCTCTCTGGC | 59.44 | 59.82 | 279 | 110 |
100.00%
|
100.00%
|
| Primer 305 | Anaerocolumna aminovalerica | TGTGCCAGCGGAAGTTCA | ACGGCATTTCGGAACCCA | 59.49 | 59.57 | 248 | 110 |
100.00%
|
100.00%
|
| Primer 306 | Anaerocolumna aminovalerica | ATCTCAGCCGTCAACCTGC | ACAGAAAGGAGAGCACCACG | 60.08 | 59.97 | 216 | 110 |
100.00%
|
100.00%
|
| Primer 307 | Anaerocolumna aminovalerica | AGGGAGCGGTTATTGGCAG | GCCGTAATGTCGTTCCAAGC | 59.78 | 59.90 | 235 | 109 |
100.00%
|
100.00%
|
| Primer 308 | Anaerocolumna aminovalerica | CATGCTCAGTTGCTTCAGGC | AGACATTGCTCCGCTTCGA | 59.83 | 59.41 | 228 | 109 |
100.00%
|
100.00%
|
| Primer 309 | Anaerocolumna aminovalerica | AGCAGCCACAAGACGCAA | CAGGCCTCCACATCAACGA | 60.20 | 59.70 | 267 | 109 |
100.00%
|
100.00%
|
| Primer 310 | Anaerocolumna aminovalerica | AGAAGCGAAACGTGCCGA | GCTGCACGGTTAGATAACAGC | 59.97 | 59.67 | 270 | 109 |
100.00%
|
100.00%
|