| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3141 | Anaerocolumna aminovalerica | TTACGGCACTTGGAGACGG | TGCAGCAATGGCAGATGC | 59.71 | 59.11 | 257 | 30 |
100.00%
|
100.00%
|
| Primer 3142 | Anaerocolumna aminovalerica | GGAGCTGGTGTTGCCGTAT | GGATGCTCACCAACTGCCT | 60.08 | 60.00 | 239 | 30 |
100.00%
|
100.00%
|
| Primer 3143 | Anaerocolumna aminovalerica | TGCTGCAATGCTGGATGC | GCCACGTACCGCTTCCATA | 59.11 | 59.86 | 202 | 30 |
100.00%
|
100.00%
|
| Primer 3144 | Anaerocolumna aminovalerica | TTGCTGCAATGCTGGATGC | GCCACGTACCGCTTCCATA | 59.78 | 59.86 | 203 | 30 |
100.00%
|
100.00%
|
| Primer 3145 | Anaerocolumna aminovalerica | CCATGCCGTAGTTCTGCCT | GCGGATCCGTGTCCCATAA | 59.78 | 59.56 | 219 | 29 |
100.00%
|
100.00%
|
| Primer 3146 | Anaerocolumna aminovalerica | GCATCCCCTTTCGGAGGTAA | ACAGCCTGCGGTAAAATGTT | 59.46 | 58.38 | 293 | 29 |
100.00%
|
100.00%
|
| Primer 3147 | Anaerocolumna aminovalerica | GCATCCCCTTTCGGAGGTAA | AACAGCCTGCGGTAAAATGT | 59.46 | 58.38 | 294 | 29 |
100.00%
|
100.00%
|
| Primer 3148 | Anaerocolumna aminovalerica | ACCGGTGGTCTTGCAGAAG | CCAGCCACTGCACAGGTAA | 59.93 | 59.93 | 215 | 29 |
100.00%
|
100.00%
|
| Primer 3149 | Anaerocolumna aminovalerica | CGGTCACTTCCGGATGATG | TGCCTGGCAGGAAAGGTAA | 58.02 | 58.84 | 190 | 29 |
100.00%
|
100.00%
|
| Primer 3150 | Anaerocolumna aminovalerica | GGGAACCGGAGTTTTCCACA | CCTGCTGTCCGGACACAAT | 60.18 | 60.00 | 170 | 28 |
100.00%
|
100.00%
|