| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Anoxybacillus flavithermus | TGGCGTCCCAACAACGAA | GGCTTCGTTAGTCTGCGGA | 59.81 | 59.79 | 252 | 118 |
100.00%
|
100.00%
|
| Primer 42 | Anoxybacillus flavithermus | TGGCGTCCCAACAACGAA | TCGTTTTGCCCACACAGGA | 59.81 | 59.78 | 223 | 118 |
100.00%
|
100.00%
|
| Primer 43 | Anoxybacillus flavithermus | TGCGTGAAGAAGTCGGCA | ATTTCGGGATCGAGCGCA | 59.58 | 59.50 | 289 | 118 |
100.00%
|
100.00%
|
| Primer 44 | Anoxybacillus flavithermus | TCGCGTCGATGTGCATGA | AGCCTGCGCTTTCGGAAT | 59.82 | 60.05 | 286 | 118 |
100.00%
|
100.00%
|
| Primer 45 | Anoxybacillus flavithermus | AATCGGTGTGCTCGTGCT | ATGCAACGGGAATGCGGA | 59.66 | 60.05 | 164 | 118 |
100.00%
|
100.00%
|
| Primer 46 | Anoxybacillus flavithermus | TCTCGCAGCAACCGAAACA | TTTTCAACGACCCCTCCCG | 60.23 | 59.93 | 175 | 115 |
100.00%
|
100.00%
|
| Primer 47 | Anoxybacillus flavithermus | CGCAGCAACCGAAACATGT | TTTTCAACGACCCCTCCCG | 59.72 | 59.93 | 172 | 115 |
100.00%
|
100.00%
|
| Primer 48 | Anoxybacillus flavithermus | CTCGCAGCAACCGAAACA | TTTTCAACGACCCCTCCCG | 58.67 | 59.93 | 174 | 115 |
100.00%
|
100.00%
|
| Primer 49 | Anoxybacillus flavithermus | TCGCAGCAACCGAAACATG | TTTTCAACGACCCCTCCCG | 59.43 | 59.93 | 173 | 115 |
100.00%
|
100.00%
|
| Primer 50 | Anoxybacillus flavithermus | TCTCGCAGCAACCGAAAC | TTTTCAACGACCCCTCCCG | 58.36 | 59.93 | 175 | 115 |
100.00%
|
100.00%
|