| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1951 | Azotobacter vinelandii | ATGACAACTGCCAGCGCA | AGGGGTTTTCCTGCAGGTG | 60.28 | 59.85 | 276 | 50 |
100.00%
|
100.00%
|
| Primer 1952 | Azotobacter vinelandii | ATCAGCCTCGAGAACAGCC | AGTCGTTGAAGCGCTGCA | 59.48 | 60.28 | 190 | 50 |
100.00%
|
100.00%
|
| Primer 1953 | Azotobacter vinelandii | ACGATCCGGTCACTTGCA | ACGCATGACTCAGCAGCA | 58.94 | 59.66 | 162 | 50 |
100.00%
|
100.00%
|
| Primer 1954 | Azotobacter vinelandii | GACGATCCGGTCACTTGCA | ACGCATGACTCAGCAGCA | 60.08 | 59.66 | 163 | 50 |
100.00%
|
100.00%
|
| Primer 1955 | Azotobacter vinelandii | CTGGGCATTCGCGTCAATG | ACGAATCGTTGCAGGCGA | 59.94 | 60.05 | 172 | 50 |
100.00%
|
100.00%
|
| Primer 1956 | Azotobacter vinelandii | GCCATCCCAAGCTGATCGA | ACGAATCGTTGCAGGCGA | 59.85 | 60.05 | 255 | 50 |
100.00%
|
100.00%
|
| Primer 1957 | Azotobacter vinelandii | TGTGGCCATGAGCGATGA | TGGCCTGTTCCTGCATCAG | 59.01 | 60.00 | 161 | 50 |
100.00%
|
100.00%
|
| Primer 1958 | Azotobacter vinelandii | TGCTGGTCGTGTTACGCA | AACCCTTGCCGAGCATCA | 59.58 | 59.24 | 162 | 50 |
100.00%
|
100.00%
|
| Primer 1959 | Azotobacter vinelandii | TACGCCAGGAAATCCGCA | TTGCATGGCACGCTCGA | 59.02 | 60.01 | 155 | 50 |
100.00%
|
100.00%
|
| Primer 1960 | Azotobacter vinelandii | CACATCCGTTCGGTGGTCA | AACTGCTCGGCATGGTGT | 60.01 | 59.57 | 197 | 50 |
100.00%
|
100.00%
|