| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bacillus paralicheniformis | AAAGACGGGTGCCATCAGG | ATCGTGGACGTGCTCAAGG | 60.00 | 60.08 | 165 | 156 |
100.00%
|
100.00%
|
| Primer 2 | Bacillus paralicheniformis | AAAGACGGGTGCCATCAGG | GGGTCCGCATCCACTTCAT | 60.00 | 59.78 | 251 | 149 |
100.00%
|
100.00%
|
| Primer 3 | Bacillus paralicheniformis | TCGTAAGAGCTGGCCAATGG | TTTGCCCAGAAGCGGTGT | 60.11 | 59.81 | 161 | 148 |
100.00%
|
100.00%
|
| Primer 4 | Bacillus paralicheniformis | AGAGCTGGCCAATGGGAT | TTTGCCCAGAAGCGGTGT | 58.23 | 59.81 | 156 | 141 |
100.00%
|
100.00%
|
| Primer 5 | Bacillus paralicheniformis | AAGAGCTGGCCAATGGGAT | TTTGCCCAGAAGCGGTGT | 58.99 | 59.81 | 157 | 141 |
100.00%
|
100.00%
|
| Primer 6 | Bacillus paralicheniformis | AAGAGCTGGCCAATGGGA | TTTGCCCAGAAGCGGTGT | 58.49 | 59.81 | 157 | 140 |
100.00%
|
100.00%
|
| Primer 7 | Bacillus paralicheniformis | TTGTTGAACCGCAGCAGC | TCTCCGTTGCCGTCATCTTC | 59.28 | 60.11 | 166 | 140 |
100.00%
|
100.00%
|
| Primer 8 | Bacillus paralicheniformis | AGCTGAAAGACAGGCTTGCA | ATTTTCGCTGCGCTTGCC | 60.18 | 60.13 | 191 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Bacillus paralicheniformis | GCTGAAAGACAGGCTTGCA | ATTTTCGCTGCGCTTGCC | 58.67 | 60.13 | 190 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Bacillus paralicheniformis | TCCTTGTGCCGATTGCCA | CTGGACAGCCTGCATGACA | 59.57 | 60.00 | 263 | 135 |
100.00%
|
100.00%
|