| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1471 | Bacillus smithii | AGGCCAGAGGACAGCTTT | TCGGCATCGGATTGGCTT | 58.09 | 59.41 | 219 | 53 |
100.00%
|
100.00%
|
| Primer 1472 | Bacillus smithii | GAGGCCAGAGGACAGCTTT | ATCGGCATCGGATTGGCT | 59.32 | 59.17 | 221 | 53 |
100.00%
|
100.00%
|
| Primer 1473 | Bacillus smithii | TCGTACCGCTGGCAAACA | AGTGCCGTTTGCGTCGA | 59.58 | 59.93 | 164 | 53 |
100.00%
|
100.00%
|
| Primer 1474 | Bacillus smithii | TTCTGCTGATGGCTGCGA | CAGCGTAAAATCCGGTGCG | 59.34 | 59.94 | 179 | 53 |
100.00%
|
100.00%
|
| Primer 1475 | Bacillus smithii | TTCTGCTGATGGCTGCGA | GGACACCACGTAGCCCAAA | 59.34 | 59.93 | 262 | 53 |
100.00%
|
100.00%
|
| Primer 1476 | Bacillus smithii | TTCTGCTGATGGCTGCGA | GCGTAAAATCCGGTGCGT | 59.34 | 58.83 | 177 | 53 |
100.00%
|
100.00%
|
| Primer 1477 | Bacillus smithii | TTCTGCTGATGGCTGCGA | AGCGTAAAATCCGGTGCGT | 59.34 | 60.38 | 178 | 53 |
100.00%
|
100.00%
|
| Primer 1478 | Bacillus smithii | AGCAAAACTGGCCTGCCA | AATGGTCAAGCCGAGCGA | 60.12 | 59.34 | 173 | 53 |
100.00%
|
100.00%
|
| Primer 1479 | Bacillus smithii | TGGACCCATGCCTGCTTT | GCACCTCGGCTCGTAACTT | 59.15 | 60.08 | 175 | 53 |
100.00%
|
100.00%
|
| Primer 1480 | Bacillus smithii | AGTGGCGAACCGTCAGTTT | AGGCTTTCTCGCAATCACCA | 59.85 | 59.96 | 199 | 53 |
100.00%
|
100.00%
|