| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 271 | Bacillus smithii | CCAGACGATCGCAGTGACA | ATTCCGGAATCCCTCGATGC | 59.79 | 59.96 | 171 | 104 |
100.00%
|
100.00%
|
| Primer 272 | Bacillus smithii | CCAGACGATCGCAGTGACA | TCGATGCTTTGACGACGGC | 59.79 | 61.09 | 158 | 104 |
100.00%
|
100.00%
|
| Primer 273 | Bacillus smithii | AGTGCGGTCGATGCGAAA | TCGCTTGTCAAGGACGTGG | 60.05 | 60.30 | 251 | 104 |
100.00%
|
100.00%
|
| Primer 274 | Bacillus smithii | AGTGCGGTCGATGCGAAA | TCCCTCGCTTGTCAAGGAC | 60.05 | 59.33 | 255 | 104 |
100.00%
|
100.00%
|
| Primer 275 | Bacillus smithii | AGTGCGGTCGATGCGAAA | GTCCCTCGCTTGTCAAGGA | 60.05 | 59.33 | 256 | 104 |
100.00%
|
100.00%
|
| Primer 276 | Bacillus smithii | AGTGCGGTCGATGCGAAA | AGTCCCTCGCTTGTCAAGG | 60.05 | 59.33 | 257 | 104 |
100.00%
|
100.00%
|
| Primer 277 | Bacillus smithii | CGCTGACGCTGCTGAAAA | TTCAACGCCAGCTCCAACT | 59.06 | 59.85 | 220 | 104 |
100.00%
|
100.00%
|
| Primer 278 | Bacillus smithii | TGGCAACCCCTGTTTCTGT | GTCAACGCAATGTGTGGACA | 59.38 | 59.34 | 197 | 104 |
100.00%
|
100.00%
|
| Primer 279 | Bacillus smithii | TGAACGTTCCCTGCCGAAA | CCTTTCCAATGCAGCGCA | 59.85 | 59.04 | 203 | 104 |
100.00%
|
100.00%
|
| Primer 280 | Bacillus smithii | CCAGACGATCGCAGTGACA | CCGGAATCCCTCGATGCTT | 59.79 | 59.55 | 168 | 103 |
100.00%
|
100.00%
|