| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bacillus subtilis | ACAGGCTGCTGATTTTGTGC | GTTGCCGAACTGCAGAAGC | 59.68 | 60.08 | 160 | 118 |
100.00%
|
100.00%
|
| Primer 2 | Bacillus subtilis | ACAGGCTGCTGATTTTGTGC | TTGCCGAACTGCAGAAGC | 59.68 | 58.66 | 159 | 118 |
100.00%
|
100.00%
|
| Primer 3 | Bacillus subtilis | TTGTGTCAGCGCTTGTGC | ATTGCTGCGTACCCTCCTG | 59.28 | 59.78 | 180 | 118 |
100.00%
|
100.00%
|
| Primer 4 | Bacillus subtilis | TTGTGTCAGCGCTTGTGC | TTCCCGACTCCGTTGACAC | 59.28 | 59.64 | 270 | 118 |
100.00%
|
100.00%
|
| Primer 5 | Bacillus subtilis | AGTAGCGCACGTGCAAGT | GCGGAATGTCGGTCAGCAT | 59.97 | 60.82 | 218 | 99 |
100.00%
|
100.00%
|
| Primer 6 | Bacillus subtilis | AGTAGCGCACGTGCAAGT | CGGAATGTCGGTCAGCATG | 59.97 | 58.99 | 217 | 99 |
100.00%
|
100.00%
|
| Primer 7 | Bacillus subtilis | AGTAGCGCACGTGCAAGT | TGACCATGCCGAATCCCTTC | 59.97 | 60.11 | 263 | 99 |
100.00%
|
100.00%
|
| Primer 8 | Bacillus subtilis | AGACGGCTTCAACGAAGAGG | ACGCACAGCCGAAATTGC | 60.04 | 59.74 | 200 | 99 |
100.00%
|
100.00%
|
| Primer 9 | Bacillus subtilis | CCTGAAAACAGCTGTCGATGC | TCAACCGGTCGAACCATCC | 60.14 | 59.71 | 191 | 99 |
100.00%
|
100.00%
|
| Primer 10 | Bacillus subtilis | TTGTGTCAGCGCTTGTGC | TCTGTTCCCGACTCCGTTG | 59.28 | 59.34 | 274 | 97 |
100.00%
|
100.00%
|