| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Bacillus subtilis | TAACGCTCGTCAGTCCGGA | TTGCCAAGCTCAGCGTCA | 60.67 | 59.89 | 214 | 82 |
100.00%
|
100.00%
|
| Primer 52 | Bacillus subtilis | TTGTGTCAGCGCTTGTGC | TCCCGACTCCGTTGACACT | 59.28 | 60.53 | 269 | 82 |
100.00%
|
100.00%
|
| Primer 53 | Bacillus subtilis | ACGGAGCACATCGCATGT | AGATTCGGCTGCTTGGACA | 59.73 | 59.32 | 213 | 81 |
100.00%
|
100.00%
|
| Primer 54 | Bacillus subtilis | AGCCCATCCACGCGAAAA | TACCCTACTGCAAGCGGTG | 59.97 | 59.41 | 220 | 81 |
100.00%
|
100.00%
|
| Primer 55 | Bacillus subtilis | AGTAGCGCACGTGCAAGT | TGACCATGCCGAATCCCTT | 59.97 | 59.00 | 263 | 81 |
100.00%
|
100.00%
|
| Primer 56 | Bacillus subtilis | TTGTGTCAGCGCTTGTGC | GTTCCCGACTCCGTTGACA | 59.28 | 59.64 | 271 | 81 |
100.00%
|
100.00%
|
| Primer 57 | Bacillus subtilis | GGCCAGCACTTCACGATTG | CGTGCACCACATTTACCGC | 59.50 | 60.15 | 154 | 81 |
100.00%
|
100.00%
|
| Primer 58 | Bacillus subtilis | ACGTAAGCGAACGCCTGT | AGTGCAGCCCAAAGCCAA | 59.66 | 60.12 | 188 | 80 |
100.00%
|
50.00%
|
| Primer 59 | Bacillus subtilis | ATAACGCTCGTCAGTCCGG | TTGCCAAGCTCAGCGTCA | 59.57 | 59.89 | 215 | 80 |
100.00%
|
100.00%
|
| Primer 60 | Bacillus subtilis | TTTGTGTCAGCGCTTGTGC | ATTGCTGCGTACCCTCCTG | 59.94 | 59.78 | 181 | 80 |
100.00%
|
100.00%
|