| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Bacillus timonensis | AGCACAATCGTTACATGGGGA | GCGTCATCAAGTGCACAGT | 59.72 | 58.76 | 215 | 140 |
100.00%
|
100.00%
|
| Primer 12 | Bacillus timonensis | AGCACAATCGTTACATGGGGA | CGTCATCAAGTGCACAGTGG | 59.72 | 59.49 | 214 | 140 |
100.00%
|
100.00%
|
| Primer 13 | Bacillus timonensis | AGCACAATCGTTACATGGGGA | ACGTTGTCAGTGCGTCATC | 59.72 | 58.48 | 226 | 140 |
100.00%
|
100.00%
|
| Primer 14 | Bacillus timonensis | ATGAGCTCATGGTGCACCC | TTGTGCCAATTGGGGAGC | 60.08 | 58.21 | 162 | 140 |
100.00%
|
100.00%
|
| Primer 15 | Bacillus timonensis | ATGAGCTCATGGTGCACCC | ATTGTGCCAATTGGGGAGC | 60.08 | 58.71 | 163 | 140 |
100.00%
|
100.00%
|
| Primer 16 | Bacillus timonensis | AGCACAATCGTTACATGGGGA | CAGTGCGTCATCAAGTGCA | 59.72 | 58.76 | 219 | 138 |
100.00%
|
100.00%
|
| Primer 17 | Bacillus timonensis | AGCACAATCGTTACATGGGGA | TCAGTGCGTCATCAAGTGCA | 59.72 | 60.25 | 220 | 138 |
100.00%
|
100.00%
|
| Primer 18 | Bacillus timonensis | ATGAGCTCATGGTGCACCC | TTGTGCCAATTGGGGAGCC | 60.08 | 60.91 | 162 | 138 |
100.00%
|
100.00%
|
| Primer 19 | Bacillus timonensis | TGCCGTCGACTTGAAGCA | GGCCAAGCTTTTCTCCACC | 59.58 | 59.03 | 160 | 138 |
100.00%
|
100.00%
|
| Primer 20 | Bacillus timonensis | TGCCGTCGACTTGAAGCA | CTGGGGTTTTCGCATTTGCA | 59.58 | 59.97 | 190 | 138 |
100.00%
|
100.00%
|